PrionDB: Pairwise Alignments

   2005, PrionDB.


The pairwise alignment has been generated with the "genewise" program of the Wise2 package. The output shows the protein sequence on the first line, followed by a line indicating the similarity level of the match, followed by 4 lines representing the DNA sequence; The DNA sequence descending in triplets, each triplet being a codon. The translation of each codon is shown above it.
Between the two protein sequences a line indicating the similarity of the match is printed. "+" indicates a conservative mismatch while "- "indicates a dramatic one. Mismatches are indicated by "***" in the left column in addition to "!" at the relevant position(s).


genewise $Name: wise2-2-0 $ (unreleased release)
This program is freely distributed under a GPL. See source directory
Copyright (c) GRL limited: portions of the code are from separate copyright

Query protein:       PRIO_TRIVU
Comp Matrix:         blosum62.bla
Gap open:            12
Gap extension:       2
Start/End            default
Target Sequence      TVPRP_5
Strand:              forward
Start/End (protein)  default
Gene Paras:          human.gf
Codon Table:         codon.table
Subs error:          1e-05
Indel error:         1e-05
Model splice?        model
Model codon bias?    flat
Model intron bias?   tied
Null model           syn
Algorithm            623

genewise output
Score 685.09 bits over entire alignment
Scores as bits over a synchronous coding model

Warning: The bits scores is not probablistically correct for single seqs
See WWW help for more info.

PRIO_TRIVU         1 MGKIQLGYWILVLFIVTWSDLGLCKKPKPRPGGGWNSGGSNRYPGQPGS 
                     MGKIQLGYWILVLFIVTWSDLGLCKKPKPRPGGGWNSGGSNRYPGQPGS 
                     MGKIQLGYWILVLFIVTWSDLGLCKKPKPRPGGGWNSGGSNRYPGQPGS 
TVPRP_5            1 agaactgttatgctagatagcgctaacacacgggtaaggaactcgccga 
                     tgatatgagtttttttcggatgtgaacacgcggggaggggagacgacgg 
                     gaacagacgcgtccttcgttacctgaagaacagggccaaccccacgttc 


PRIO_TRIVU        50 PGGNRYPGWGHPQGGGTNWGQPHPGGSNWGQPHPGGSSWGQPHGGSNWG 
                     PGGNRYPGWGHPQGGGTNWGQPHPGGSNWGQPHPGGSSWGQPHGGSNWG 
                     PGGNRYPGWGHPQGGGTNWGQPHPGGSNWGQPHPGGSSWGQPHGGSNWG 
TVPRP_5          148 cggactcgtgcccgggaatgccccggtatgccccggtatgcccggtatg 
                     cggagacgggacagggcaggacacggcaggacacggcgggacaggcagg 
                     taccactcggctgatcctgtgctcgtctgtgcttgtctgtgattatcgt 


PRIO_TRIVU        99 QGGYNKWKPDKPKTNLKHVAGAAAAGAVVGGLGGYMLGSAMSRPVIHFG 
                     QGGYNKWKPDKPKTNLKHVAGAAAAGAVVGGLGGYMLGSAMSRPVIHFG 
                     QGGYNKWKPDKPKTNLKHVAGAAAAGAVVGGLGGYMLGSAMSRPVIHFG 
TVPRP_5          295 cggtaatacgacaaacacgggggggggggggcggtacgagaaacgactg 
                     aggaaagacaacacataatcgccccgcttggtggattggctggcttatg 
                     gccccggaatacaccaatgtactttgatgagttccgggtcgcgccactc 


PRIO_TRIVU       148 NEYEDRYYRENQYRYPNQVMYRPIDQYSSQNNFVHDCVNITVKQHTTTT 
                     NEYEDRYYRENQYRYPNQVMYRPIDQYSSQNNFVHDCVNITVKQHTTTT 
                     NEYEDRYYRENQYRYPNQVMYRPIDQYSSQNNFVHDCVNITVKQHTTTT 
TVPRP_5          442 agtggcttcgactctcacgatccagctaacaatgcgtgaaagaccaaaa 
                     aaaaagaagaaaagacaattagctaaaggaaattaagtatctaaacccc 
                     tacaccctaacgcccccacgcgcccgcccgcttgtcccccggaaccccc 


PRIO_TRIVU       197 TTKGENFTETDIKIMERVVEQMCITQYQAEYEAAAQRAYNMAFFSAPPV 
                     TTKGENFTETDIKIMERVVEQMCITQYQAEYEAAAQRAYNMAFFSAPPV 
                     TTKGENFTETDIKIMERVVEQMCITQYQAEYEAAAQRAYNMAFFSAPPV 
TVPRP_5          589 aaaggatagagaaaagcgggcataactcggtggggccgtaagtttgccg 
                     ccagaatcacatattagttaatgtcaaacaaacccagcaatcttcccct 
                     ccaggccaattcgtggtgggggccggcatgcgtctgcgccgacctcgag 


PRIO_TRIVU       246 TLLFLSFLIFLIVS                                    
                     TLLFLSFLIFLIVS                                    
                     TLLFLSFLIFLIVS                                    
TVPRP_5          736 acctcatcatcaga                                    
                     cttttgtttttttg                                    
                     cccccccttcgtgc                                    


//


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F.Horn (priondbcmbi.ru.nl), 18-Aug-2005