PrionDB: Extraction of mutation data from the literature

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This data was extracted from Medline abstracts and full texts (when available) in an automated manner.

The table below describes different point mutations at a given position and provides links to other documents. The sentence(s) where the point mutations in PRIO_MOUSE at position 108 were found are listed after the table.

Point mutations at position L108 in PRIO_MOUSE

ProteinPRIO_MOUSE (P04925)    Gene: Prnp,Prn-p    (other point mutations)Swiss-Prot
Cross-reference table
Family page
PositionL108
General numbering (PrionDB) -
DomainNot determined
Family alignments Mammalian prion proteins
Prion proteins (PRP, PRNP)
Other point mutations at the same position Position 109 in Mammalian prion proteins family
Position 109 in Prion proteins (PRP, PRNP) family
Reference #1Liemann S, Glockshuber R
Biochemistry 1999 Mar 16;38(11):3258-67.		Medline
Text sourceHTML and PDF full texts
Point mutationL108F (True positive)
Cited point mutationL109F,Leu109Phe
Reference #2Manson JC, Jamieson E, Baybutt H, Tuzi NL, Barron R, McConnell I, Somerville R, Ironside J, Will R, Sy MS, Melton DW, Hope J, Bostock C
EMBO J 1999 Dec 1;18(23):6855-64.		Medline
Text sourceHTML full text
Point mutationL108F (Not yet checked)
Reference #3Walmsley AR, Zeng F, Hooper NM
EMBO J 2001 Feb 15;20(4):703-12.		Medline
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Point mutationL108M (Not yet checked)
Reference #4Holscher C, Bach UC, Dobberstein B
J Biol Chem 2001 Apr 20;276(16):13388-94.		Medline
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Point mutationL108M (True positive)
Reference #5Mastrangelo P, Westaway D
Gene 2001 Sep 5;275(1):1-18.		Medline
Text sourcePDF full text
Point mutationL108F (Not yet checked)
Reference #6Atarashi R, Nishida N, Shigematsu K, Goto S, Kondo T, Sakaguchi S, Katamine S
J Biol Chem 2003 Aug 1;278(31):28944-9. Epub 2003 May 19.		Medline
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Point mutationL108M (True positive)
Reference #7Heller U, Winklhofer KF, Heske J, Reintjes A, Tatzelt J
J Biol Chem 2003 Sep 19;278(38):36139-47. Epub 2003 Jul 8.		Medline
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Point mutationL108M (True positive)
Reference #8Heske J, Heller U, Winklhofer KF, Tatzelt J
J Biol Chem 2004 Feb 13;279(7):5435-43. Epub 2003 Nov 26.		Medline
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Point mutationL108M (Not yet checked)
Cited point mutationLeu108Met,Leu108
Reference #9Tremblay P, Ball HL, Kaneko K, Groth D, Hegde RS, Cohen FE, DeArmond SJ, Prusiner SB, Safar JG
J Virol 2004 Feb;78(4):2088-99.		Medline
Text sourcePDF full text
Point mutationL108F (True positive)

Relevant sentences

Reference #2 (Manson JC et al.): L108F
  • Additional evidence that suggests that the alteration in PrP protein level may be a specific effect of the 101L mutation is provided by the previous gene targeting experiments in which we introduced alterations into amino acids L108F and T189V

Reference #5 (Mastrangelo P et al.): L108F
  • The ability of Prnp0 / 0 mice to replicate prions is re Table 1 PrP gene polymorphisms that modulate the progress of prion infections Codon(s) Species (prevalent subgroup) Disease affected L108F , T189V Mouse Experimental scrapie (Westaway et al. , 1987) M129V Human (all ethnic groups) Iatrogenic CJD , sporadic CJD (FFI vs

Reference #9 (Tremblay P et al.): L108F
  • Taken together , our findings argue that the P101L mutation has an effect on incubation times similar to that of the L108F and T189V substitutions encoded in mouse Prnpb (49)

Reference #3 (Walmsley AR et al.): L108M
  • Wild-type murine PrP and the constructs had methionine residues substituted at the two positions indicated (L108M and V111M) , which allowed the proteins to be recognized by the monoclonal antibody 3F4

Reference #4 (Holscher C et al.): L108M
  • The 3F4 epitope (L108M / V111M) was introduced by using the QuikChange TM site-directed mutagenesis kit (Stratagene)

Reference #6 (Atarashi R et al.): L108M
  • MHM2 PrP has two amino acid substitutions from Mo.PrP , L108M and V111M , which are included in the epitope recognized by 3F4 anti-PrP antibody

  • MH2M PrP has five amino acid substitutions (L108M , V111M , I138M , Y154N , and S169N) from Mo.PrP

Reference #7 (Heller U et al.): L108M
  • PrP sequences were amplified from pcDNA3.1-3F4 (35 ) , which contains the mouse PRNP modified to express PrP L108M / V111M for im munostaining with the monoclonal antibody 3F4

Reference #8 (Heske J et al.): Leu108
  • EXPERIMENTAL PROCEDURES (image)TOP (image)ABSTRACT (image)INTRODUCTION (image)EXPERIMENTAL PROCEDURES (image)RESULTS (image)DISCUSSION (image)REFERENCES Generation of PrP Mutants—For expression of wild-type PrP pcDNA3.1-3F4 was generated by cloning the open reading frame of the mouse PRNP gene into the pcDNA3.1 vector ; Leu108 and Val111 were replaced by Met to allow detection by the 3F4 monoclonal antibody (29 )

Reference #1 (Liemann S et al.): Leu109Phe
  • No significant influence on protein stability is also expected for the replacement Leu109Phe , since residue 109 is located in the unstructured N-terminal segment of mPrP C

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F.Horn (priondbcmbi.ru.nl), 22-Aug-2005