PrionDB: Extraction of mutation data from the literature

2005, PrionDB.

This data was extracted from Medline abstracts and full texts (when available) in an automated manner.

The table below describes the selected point mutation and provides links to other documents. The sentence(s) where the point mutation D147N was found are listed after the table.

The mutated residues are also indicated in the family sequence alignments and hyperlinked to the corresponding mutation pages.

Point mutation D147N in PRIO_CRIGR

Point mutation:	D147N
Domain:	Not determined
General numbering (PrionDB):	-
Protein:	PRIO_CRIGR (PRNP)	Swiss-Prot Cross-reference table Family page
Other point mutations / same protein	List of mutations in PRIO_CRIGR
Family alignments	Mammalian prion proteins Prion proteins (PRP, PRNP)
Other point mutations / same position	Position 147 in Mammalian prion proteins family Position 147 in Prion proteins (PRP, PRNP) family
Reference:	From conversion to aggregation: protofibril formation of the prion protein. DeMarco ML, Daggett V Proc Natl Acad Sci U S A 2004 Feb 24;101(8):2293-8.	Medline
Other point mutations / same article	List
Text source	HTML full text
Validation status	Not yet checked

Relevant sentences:

D147N

Motivated by experimental results from the Caughey group (21 ) , we performed si mulations of Syrian hamster PrP , residues 109-219 (5 ) , with the D147N mutation (hamster numbering is used throughout this article)
Here , we describe the dynamic behavior of D147N in water at both neutral and low pH levels
The D147N mutation disrupted the native conformation of the N-terminal residues 109-170
Si mulated conversion of Syrian hamster D147N PrP C to PrP Sc at low pH levels
Both the neutral and low pH si mulations of the D147N mutant yielded structures that deviated significantly from the starting NMR structure

F.Horn (priondbcmbi.ru.nl), 22-Aug-2005