NucleaRDB: Pairwise Alignments

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The pairwise alignment has been generated with the "genewise" program of the Wise2 package. The output shows the protein sequence on the first line, followed by a line indicating the similarity level of the match, followed by 4 lines representing the DNA sequence; The DNA sequence descending in triplets, each triplet being a codon. The translation of each codon is shown above it.
Between the two protein sequences a line indicating the similarity of the match is printed. "+" indicates a conservative mismatch while "- "indicates a dramatic one. Mismatches are indicated by "***" in the left column in addition to "!" at the relevant position(s).


genewise $Name: wise2-2-0 $ (unreleased release)
This program is freely distributed under a GPL. See source directory
Copyright (c) GRL limited: portions of the code are from separate copyright

Query protein:       STF1_MACEU
Comp Matrix:         blosum62.bla
Gap open:            12
Gap extension:       2
Start/End            default
Target Sequence      AF401742_2
Strand:              forward
Start/End (protein)  default
Gene Paras:          human.gf
Codon Table:         codon.table
Subs error:          1e-05
Indel error:         1e-05
Model splice?        model
Model codon bias?    flat
Model intron bias?   tied
Null model           syn
Algorithm            623

genewise output
Score 1179.03 bits over entire alignment
Scores as bits over a synchronous coding model

Warning: The bits scores is not probablistically correct for single seqs
See WWW help for more info.

STF1_MACEU         1 MDYSYDEDLDELCPVCGDKVSGYHYGLLTCESCKGFFKRTVQNNKHYTC 
                     MDYSYDEDLDELCPVCGDKVSGYHYGLLTCESCKGFFKRTVQNNKHYTC 
                     MDYSYDEDLDELCPVCGDKVSGYHYGLLTCESCKGFFKRTVQNNKHYTC 
AF401742_2         1 agtttgggcggctcgtggagtgtctgccatgatagttacagcaaactat 
                     taacaaaataatgctggaatcgaaagttcgaggagttagctaaaaaacg 
                     gctgccgcgcgacggcacagcacctggcccgctggctgcgagtcaccgc 


STF1_MACEU        50 TESQNCKIDKTQRKRCPYCRFQKCLTVGMRLEAVRADRMRGGRNKFGPM 
                     TESQNCKIDKTQRKRCPYCRFQKCLTVGMRLEAVRADRMRGGRNKFGPM 
                     TESQNCKIDKTQRKRCPYCRFQKCLTVGMRLEAVRADRMRGGRNKFGPM 
AF401742_2       148 agacataagaaccactcttctcatcaggaccgggcggcacggaaatgca 
                     cagaagataacagaggcaggtaagtctgtgtactgcagtggggaatgct 
                     cgcgccgtcgagtaaccccccggcgtgggggattgacaggtaacgttcg 


STF1_MACEU        99 YKRDRALKQQKKALIRANGFKLETGPSMGPPPQTDYPLAPALHPGAKGL 
                     YKRDRALKQQKKALIRANGFKLETGPSMGPPPQTDYPLAPALHPGAKGL 
                     YKRDRALKQQKKALIRANGFKLETGPSMGPPPQTDYPLAPALHPGAKGL 
AF401742_2       295 taagcgtaccaagcacgagtacgagctagccccagtctgcgcccggagt 
                     aagagctaaaaacttgcagtatacgcctgcccacaactccctacgcagt 
                     cgactcaaggggcgcactacgtggtacgattaatcccgtacccagcgcg 


STF1_MACEU       148 APAPPAGPPGDYERGPYGPPGVPMAVPTHGPLAGYLYPAFPGRAIKSEY 
                     APAPPAGPPGDYERGPYGPPGVPMAVPTHGPLAGYLYPAFPGRAIKSEY 
                     APAPPAGPPGDYERGPYGPPGVPMAVPTHGPLAGYLYPAFPGRAIKSEY 
AF401742_2       442 gcgccggccggtgcgctgccggcaggcacgctggtctcgtcgcgaatgt 
                     ccccccgccgaaaggcagccgtctctccagctcgatacctcggctacaa 
                     ataatagttactggctttaatatgggcctcagtctcctcttctccgagt 


STF1_MACEU       197 PEPYASPHEPAPPYGYPEPYPSGPGLPGVPELILKLLQLEPDEGQLKAR 
                     PEPYASPHEPAPPYGYPEPYPSGPGLPGVPELILKLLQLEPDEGQLKAR 
                     PEPYASPHEPAPPYGYPEPYPSGPGLPGVPELILKLLQLEPDEGQLKAR 
AF401742_2       589 cgctgaccgcgcctgtcgctcagcgccggcgcacaccccgcgggccagc 
                     cacacgcaaccccagacacacggcgtcgtcatttattatacaagatacg 
                     agcttcctgacactccagctttctcgagcagcccagggagttacgcgca 


STF1_MACEU       246 ILACLQEPSKGRPDRPTPFGLMCKMADQTLFSIVEWARSCVVFKELEVA 
                     ILACLQEPSKGRPDRPTPFGLMCKMADQTLFSIVEWARSCVVFKELEVA 
                     ILACLQEPSKGRPDRPTPFGLMCKMADQTLFSIVEWARSCVVFKELEVA 
AF401742_2       736 acgtccgctagccgccactgcataaggcacttaggtgcatggtagcggg 
                     ttcgtaaccaggcagccctgttgatcaacttcttagcgggtttaatatc 
                     agccaggtcgcgttgcatttcgcggttggccattagtgttgccgagggt 


STF1_MACEU       295 DQMKLLQNCWSELLVFDHIYRQIQHGKEGSILLVTGQEVDLSTVAAQAG 
                     DQMKLLQNCWSELLVFDHIYRQIQHGKEGSILLVTGQEVDLSTVAAQAG 
                     DQMKLLQNCWSELLVFDHIYRQIQHGKEGSILLVTGQEVDLSTVAAQAG 
AF401742_2       883 gcaactcattagccgtgcatacaccgaggaatcgagcgggttagggcgg 
                     aatattaagggattttaatagataagaaggttttcgaatatcctccacg 
                     cggatggtcgcggtctccccagcatggggctggctcggacaaagacgaa 


STF1_MACEU       344 SLLHSLVLRAQDLVQQLHSLQVDRQEFVCLKFLILFSLDVKFLENHGLA 
                     SLLHSLVLRAQDLVQQLHSLQVDRQEFVCLKFLILFSLDVKFLENHGLA 
                     SLLHSLVLRAQDLVQQLHSLQVDRQEFVCLKFLILFSLDVKFLENHGLA 
AF401742_2      1030 tcccacgccgcgcgcccctccggacgtgttatcactacggatcgacgcg 
                     cttagtttgcaattaatactatagaattgtatttttgtatattaaagtc 
                     ttcccagggcgctaaggctagtcggatccggccccccttggcggtctgc 


STF1_MACEU       393 KDAQEKANSALLEYTMCHYPHCGDKFRQLLLRLAEVRSLSMQAEEYLYH 
                     KDAQEKANSALLEYTMCHYPHCGDKFRQLLLRLAEVRSLSMQAEEYLYH 
                     KDAQEKANSALLEYTMCHYPHCGDKFRQLLLRLAEVRSLSMQAEEYLYH 
AF401742_2      1177 aggcgagatgccgtaatctcctggatcccccccgggctcaacgggtctc 
                     aacaaacaccttaactgaacaggaatgatttgtcatgctgtacaaataa 
                     gttaggccacgggccgctctctccgcgggcgcgtggctccggcggcgcc 


STF1_MACEU       442 KHLGGEVPCNNLLIEMLHAKRT                            
                     KHLGGEVPCNNLLIEMLHAKRT                            
                     KHLGGEVPCNNLLIEMLHAKRT                            
AF401742_2      1324 accggggctaaccagaccgaca                            
                     aatggatcgaatttattacagc                            
                     gcgtgggccccgttgggtcggt                            


//


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F.Horn (nucleardbcmbi.kun.nl), 18-Feb-2005