NucleaRDB: Pairwise Alignments

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The pairwise alignment has been generated with the "genewise" program of the Wise2 package. The output shows the protein sequence on the first line, followed by a line indicating the similarity level of the match, followed by 4 lines representing the DNA sequence; The DNA sequence descending in triplets, each triplet being a codon. The translation of each codon is shown above it.
Between the two protein sequences a line indicating the similarity of the match is printed. "+" indicates a conservative mismatch while "- "indicates a dramatic one. Mismatches are indicated by "***" in the left column in addition to "!" at the relevant position(s).


genewise $Name: wise2-2-0 $ (unreleased release)
This program is freely distributed under a GPL. See source directory
Copyright (c) GRL limited: portions of the code are from separate copyright

Query protein:       PXR_RAT
Comp Matrix:         blosum62.bla
Gap open:            12
Gap extension:       2
Start/End            default
Target Sequence      AF151377_2
Strand:              forward
Start/End (protein)  default
Gene Paras:          human.gf
Codon Table:         codon.table
Subs error:          1e-05
Indel error:         1e-05
Model splice?        model
Model codon bias?    flat
Model intron bias?   tied
Null model           syn
Algorithm            623

genewise output
Score 1078.20 bits over entire alignment
Scores as bits over a synchronous coding model

Warning: The bits scores is not probablistically correct for single seqs
See WWW help for more info.

PXR_RAT            1 MRPEERWNHVGLVQREEADSVLEEPINVDEEDGGLQICRVCGDKANGYH 
                     MRPEERWNHVGLVQREEADSVLEEPINVDEEDGGLQICRVCGDKANGYH 
                     MRPEERWNHVGLVQREEADSVLEEPINVDEEDGGLQICRVCGDKANGYH 
AF151377_2         1 aacggatacggcgccggggtgtggcaagggggggccatcgtggagagtc 
                     tgcaaggaatgttagaacacttaactataaaaggtatggtggaacagaa 
                     gatggggcttctaataaattcgagtccatgatattacctatgcgctctc 


PXR_RAT           50 FNVMTCEGCKGFFRRAMKRNVRLRCPFRKGTCEITRKTRRQCQACRLRK 
                     FNVMTCEGCKGFFRRAMKRNVRLRCPFRKGTCEITRKTRRQCQACRLRK 
                     FNVMTCEGCKGFFRRAMKRNVRLRCPFRKGTCEITRKTRRQCQACRLRK 
AF151377_2       148 tagaatggtagttaagaacagccatctcagatgaacaaccctcgtctca 
                     tattcgaggagttggctagatgtggctgagcgatcgacggagacggtga 
                     ctcgctaatgatcagcgactcgggccccggccgccggcaggcgcctgcg 


PXR_RAT           99 CLESGMKKEMIMSDAAVEQRRALIKRKKREKIEAPPPGGQGLTEEQQAL 
                     CLESGMKKEMIMSDAAVEQRRALIKRKKREKIEAPPPGGQGLTEEQQAL 
                     CLESGMKKEMIMSDAAVEQRRALIKRKKREKIEAPPPGGQGLTEEQQAL 
AF151377_2       295 tcgagaaagaaatgggggcacgtaaaaaagaaggcccggcgcaggccgc 
                     gtaggtaaatttcacctaaggcttagaagaataccccggagtcaaaact 
                     cggtcggaggcgttctgagggcgcgggggagtgtagtaggggaaagggg 


PXR_RAT          148 IQELMDAQMQTFDTTFSHFKDFRLPAVFHSDCELPEVLQASLLEDPATW 
                     IQELMDAQMQTFDTTFSHFKDFRLPAVFHSDCELPEVLQASLLEDPATW 
                     IQELMDAQMQTFDTTFSHFKDFRLPAVFHSDCELPEVLQASLLEDPATW 
AF151377_2       442 acgcaggcacatgaattctagtcccggtcagtgccggccgtctggcgat 
                     taattacatactacctcataatgtccttagagatcattaccttaacccg 
                     cggggctggactcatccccgtcgatggtctccgtggtggcaggactcag 


PXR_RAT          197 SQIMKDSVPMKISVQLRGEDGSIWNYQPPSKSDGKEIIPLLPHLADVST 
                     SQIMKDSVPMKISVQLRGEDGSIWNYQPPSKSDGKEIIPLLPHLADVST 
                     SQIMKDSVPMKISVQLRGEDGSIWNYQPPSKSDGKEIIPLLPHLADVST 
AF151377_2       589 acaaagagcaaatgcccggggaatatccctaaggagaaccccccgggta 
                     gattaagtctatctatggaaggtgaaacccagagaattcttcatcatcc 
                     tacgacttaggctgggcaaccccgccatccgccgagccccggcgctgac 


PXR_RAT          246 YMFKGVINFAKVISHFRELPIEDQISLLKGATFEMCILRFNTMFDTETG 
                     YMFKGVINFAKVISHFRELPIEDQISLLKGATFEMCILRFNTMFDTETG 
                     YMFKGVINFAKVISHFRELPIEDQISLLKGATFEMCILRFNTMFDTETG 
AF151377_2       736 tataggaatgagatctagccaggcatccaggatgatacataaatgagag 
                     attagttatcattcatgatctaaatcttagcctatgttgtacttacacg 
                     cgcgccccccacacccgggtcgcgccggggctcggccggccggccgaaa 


PXR_RAT          295 TWECGRLAYCFEDPNGGFQKLLLDPLMKFHCMLKKLQLREEEYVLMQAI 
                     TWECGRLAYCFEDPNGGFQKLLLDPLMKFHCMLKKLQLREEEYVLMQAI 
                     TWECGRLAYCFEDPNGGFQKLLLDPLMKFHCMLKKLQLREEEYVLMQAI 
AF151377_2       883 atgtgccgtttggcaggtcacccgctaatctacaaccccgggtgcacga 
                     cgagggtcagtaacaggtaatttacttatagttaatatgaaaatttact 
                     cggctggtcccacttcccggcggcgggacccggggaggtgggcggggcc 


PXR_RAT          344 SLFSPDRPGVVQRSVVDQLQERFALTLKAYIECSRPYPAHRFLFLKIMA 
                     SLFSPDRPGVVQRSVVDQLQERFALTLKAYIECSRPYPAHRFLFLKIMA 
                     SLFSPDRPGVVQRSVVDQLQERFALTLKAYIECSRPYPAHRFLFLKIMA 
AF151377_2      1030 tcttcgccgggccagggcccgctgcacagtagtacctcgcatctcaaag 
                     cttccagcgttaggttaataagtctctacatagggcaccagttttattc 
                     ccccatctcgtatcgacggggatcccggcccgttgcttacgcgcggcgt 


PXR_RAT          393 VLTELRSINAQQTQQLLRIQDTHPFATPLMQELFSSTDG           
                     VLTELRSINAQQTQQLLRIQDTHPFATPLMQELFSSTDG           
                     VLTELRSINAQQTQQLLRIQDTHPFATPLMQELFSSTDG           
AF151377_2      1177 gcagccaaagccacccccacgacctgaccacgttaaagg           
                     ttcatggtacaacaattgtaacactcccttaattggcag           
                     cccggctctcggcggagccgcgcctcatcgggacccgcc           


//


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F.Horn (nucleardbcmbi.kun.nl), 18-Feb-2005