NucleaRDB: Pairwise Alignments

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The pairwise alignment has been generated with the "genewise" program of the Wise2 package. The output shows the protein sequence on the first line, followed by a line indicating the similarity level of the match, followed by 4 lines representing the DNA sequence; The DNA sequence descending in triplets, each triplet being a codon. The translation of each codon is shown above it.
Between the two protein sequences a line indicating the similarity of the match is printed. "+" indicates a conservative mismatch while "- "indicates a dramatic one. Mismatches are indicated by "***" in the left column in addition to "!" at the relevant position(s).


genewise $Name: wise2-2-0 $ (unreleased release)
This program is freely distributed under a GPL. See source directory
Copyright (c) GRL limited: portions of the code are from separate copyright

Query protein:       DAX1_PIG
Comp Matrix:         blosum62.bla
Gap open:            12
Gap extension:       2
Start/End            default
Target Sequence      AF035816_2
Strand:              forward
Start/End (protein)  default
Gene Paras:          human.gf
Codon Table:         codon.table
Subs error:          1e-05
Indel error:         1e-05
Model splice?        model
Model codon bias?    flat
Model intron bias?   tied
Null model           syn
Algorithm            623

genewise output
Score 1173.95 bits over entire alignment
Scores as bits over a synchronous coding model

Warning: The bits scores is not probablistically correct for single seqs
See WWW help for more info.

DAX1_PIG           1 MAGEDHQWQGSILYNMLMSAKQTHATREAPEARLRGSCWGCSCGSEPPV 
                     MAGEDHQWQGSILYNMLMSAKQTHATREAPEARLRGSCWGCSCGSEPPV 
                     MAGEDHQWQGSILYNMLMSAKQTHATREAPEARLRGSCWGCSCGSEPPV 
AF035816_2         1 aggggcctcgaactaacaagacacgacggcggccagtttgtttgtgccg 
                     tcgaaaagaggttaatttgcaacaccgaccacgtggcggggcggcacct 
                     ggcgctgggccccccgcgcaaagtgagggcgagggtgcgccctcggcgg 


DAX1_PIG          50 GREGQPGGPAVALLYRCCFCGEDHPRQGSILYNMLTSAKQTQETPEAPE 
                     GREGQPGGPAVALLYRCCFCGEDHPRQGSILYNMLTSAKQTQETPEAPE 
                     GREGQPGGPAVALLYRCCFCGEDHPRQGSILYNMLTSAKQTQETPEAPE 
AF035816_2       148 gaggccggcgggcctcttttgggccacgaactaacaagacacgacggcg 
                     ggagacggcctcttagggtggaaacgaggttaattcgcaacaaccacca 
                     caggggtagaggcgctcctctaccgggccccccgcgcagaggatggccg 


DAX1_PIG          99 ARLGGACWGCSCGSEPRVGREELPGGRATVLLYRCCFCGEEHPRQGSIL 
                     ARLGGACWGCSCGSEPRVGREELPGGRATVLLYRCCFCGEEHPRQGSIL 
                     ARLGGACWGCSCGSEPRVGREELPGGRATVLLYRCCFCGEEHPRQGSIL 
AF035816_2       295 gccgggttgtttgtgccggaggccggcgagcctcttttgggccccgaac 
                     cgtggcggggcggcacgtggaatcgggcctttagggtggaaacgaggtt 
                     agcgaacgccatctgcggtagagctggcagcgcccctctggcaggcccg 


DAX1_PIG         148 YSLLTSAKQTHVALEAPEARPGGAWWDRSYCAQRLGAREELPGGRPVTL 
                     YSLLTSAKQTHVALEAPEARPGGAWWDRSYCAQRLGAREELPGGRPVTL 
                     YSLLTSAKQTHVALEAPEARPGGAWWDRSYCAQRLGAREELPGGRPVTL 
AF035816_2       442 tatcaagacacggcggcggccgggttgctttgcacggaggccggccgac 
                     agttcgcaacatctaccacgcggcggagcagcagtgcgaatcgggctct 
                     ccgcccagagcgtgatggggggcaggcccccagggacagggtcgggggc 


DAX1_PIG         197 PYRCCFCGEDHPRQSGILCNMPMSAKQTHVAPEAQPGAPWWDPSCAAQR 
                     PYRCCFCGEDHPRQSGILCNMPMSAKQTHVAPEAQPGAPWWDPSCAAQR 
                     PYRCCFCGEDHPRQSGILCNMPMSAKQTHVAPEAQPGAPWWDPSCAAQR 
AF035816_2       589 ctcttttgggccccagactaacaagacacggcggccggcttgcttggcc 
                     cagggtggaaacgaggttgatctgcaacatccacacgccggaccgccag 
                     acccctctaccaggtcccccgtgcagagcgtagggggccggccgtcggg 


DAX1_PIG         246 VALKSPQVVCEAASAGLLKTLRFVKYLPCFQVLPLDQQLVLVRSCWAPL 
                     VALKSPQVVCEAASAGLLKTLRFVKYLPCFQVLPLDQQLVLVRSCWAPL 
                     VALKSPQVVCEAASAGLLKTLRFVKYLPCFQVLPLDQQLVLVRSCWAPL 
AF035816_2       736 ggcaaccggtgggtggctaacctgattcttcgcctgcctgcgcattgcc 
                     tctagcattgaccccgttactgttaatcgtattctaaattttggggcct 
                     gccgcaggtcgacggcgggggcccgcgcccgggcgcggggtgcctgggc 


DAX1_PIG         295 LMLELAQDRLNFETVETLEPSLLQMILTTRRQETEGDEPPSPQPPVQPH 
                     LMLELAQDRLNFETVETLEPSLLQMILTTRRQETEGDEPPSPQPPVQPH 
                     LMLELAQDRLNFETVETLEPSLLQMILTTRRQETEGDEPPSPQPPVQPH 
AF035816_2       883 cacgcgcgctatgaggatgcacccaacaaaccgaggggcctccccgccc 
                     tttatcaagtatactactacgttatttccggaacagaaccccacctaca 
                     cgaggcgccgctggggcggccgaggccccggggagatggtgcgcaggac 


DAX1_PIG         344 LVLPSEAEHLPSVAEVQAIKGFLAKCWSLDISTKEYAYLKGTVLFNPDL 
                     LVLPSEAE       VQAIKGFLAKCWSLDISTKEYAYLKGTVLFNPDL 
                     LVLPSEAE-------VQAIKGFLAKCWSLDISTKEYAYLKGTVLFNPDL 
AF035816_2      1030 tgcctggg       gcgaagtcgattacgaaaagtgtcagagctacgc 
                     tttccaca       tactagttcagggtatgcaaacatagctttacat 
                     gaggggcg       caccgcctcgcgcacctcggtcccggcgcttgcg 


DAX1_PIG         393 PGLQCVKYIQGLQWGTQQILSEHIRMTHRGYQARFAELNSALFLLRFIN 
                     PGLQCVKYIQGLQWGTQQILSEHIRMTHRGYQARFAELNSALFLLRFIN 
                     PGLQCVKYIQGLQWGTQQILSEHIRMTHRGYQARFAELNSALFLLRFIN 
AF035816_2      1156 cgcctgatacgcctgaccacagcaaaacagtcgatggcaagctccataa 
                     cgtagtaatagtaggcaattgaatgtcaggaacgtcatagcttttgtta 
                     agtgcggccgatggatggctcaccggacggcgcattggccctcggacct 


DAX1_PIG         442 ANVLAELFFRPIIGTVSMDDMMLEMLCAKL                    
                     ANVLAELFFRPIIGTVSMDDMMLEMLCAKL                    
                     ANVLAELFFRPIIGTVSMDDMMLEMLCAKL                    
AF035816_2      1303 gagcggcttacaagagaaggaacgactgat                    
                     cattcatttgcttgctgtaatttattgcat                    
                     ctctcagccgcctctccgtcgggagctaga                    


//


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F.Horn (nucleardbcmbi.kun.nl), 18-Feb-2005