Swiss-Prot entry
ID PPAT_PIG STANDARD; PRT; 504 AA.
AC O62807; O77815;
DT 16-OCT-2001 (Rel. 40, Created)
DT 16-OCT-2001 (Rel. 40, Last sequence update)
DT 01-MAY-2005 (Rel. 47, Last annotation update)
DE Peroxisome proliferator activated receptor gamma (PPAR-gamma).
GN Name=PPARG; Synonyms=NR1C3;
OS Sus scrofa (Pig).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Laurasiatheria; Cetartiodactyla; Suina; Suidae;
OC Sus.
OX NCBI_TaxID=9823;
RN [1]
RP NUCLEOTIDE SEQUENCE (ISOFORMS 1 AND 2).
RC TISSUE=Adipose tissue;
RX MEDLINE=99132654; PubMed=9931452 [NCBI, ExPASy, EBI, Israel, Japan]; DOI=10.1016/S0378-1119(98)00533-2;
RA Houseknecht K.L., Bidwell C.A., Portocarrero C.P., Spurlock M.E.;
RT "Expression and cDNA cloning of porcine peroxisome proliferator-
RT activated receptor gamma (PPARgamma).";
RL Gene 225:89-96(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE (ISOFORM 2).
RX MEDLINE=99410873; PubMed=10481259 [NCBI, ExPASy, EBI, Israel, Japan]; DOI=10.1016/S0305-0491(99)00077-2;
RA Ding S.T., McNeel R.L., Mersmann H.J.;
RT "Expression of porcine adipocyte transcripts: tissue distribution and
RT differentiation in vitro and in vivo.";
RL Comp. Biochem. Physiol. 123B:307-318(1999).
RN [3]
RP NUCLEOTIDE SEQUENCE (ISOFORMS 1 AND 2).
RC STRAIN=Duroc, and Norwegian Landrace; TISSUE=Adipocyte;
RX MEDLINE=98401156; PubMed=9731203 [NCBI, ExPASy, EBI, Israel, Japan]; DOI=10.1006/bbrc.1998.9212;
RA Grindflek E., Sundvold H., Klungland H., Lien S.;
RT "Characterisation of porcine peroxisome proliferator-activated
RT receptors gamma 1 and gamma 2: detection of breed and age differences
RT in gene expression.";
RL Biochem. Biophys. Res. Commun. 249:713-718(1998).
CC -!- FUNCTION: Receptor that binds peroxisome proliferators such as
CC hypolipidemic drugs and fatty acids. Once activated by a ligand,
CC the receptor binds to a promoter element in the gene for acyl-CoA
CC oxidase and activates its transcription. It therefore controls the
CC peroxisomal beta-oxidation pathway of fatty acids. Key regulator
CC of adipocyte differentiation and glucose homeostasis.
CC -!- SUBUNIT: Forms a heterodimer with the retinoic acid receptor RXR-
CC alpha called adipocyte-specific transcription factor ARF6.
CC Interacts with NCOA6 coactivator, leading to a strong increase in
CC transcription of target genes (By similarity).
CC -!- SUBCELLULAR LOCATION: Nuclear.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=2;
CC IsoId=O62807-1; Sequence=Displayed;
CC Name=1;
CC IsoId=O62807-2; Sequence=VSP_003648;
CC -!- TISSUE SPECIFICITY: Highest expression in adipose tissue and lower
CC in spleen. Very low levels in kidney, intestine, lung and muscle.
CC -!- SIMILARITY: Belongs to the nuclear hormone receptor family. NR1
CC subfamily.
CC -!- SIMILARITY: Contains 1 nuclear receptor DNA-binding domain.
CC --------------------------------------------------------------------------
CC This Swiss-Prot entry is copyright. It is produced through a collaboration
CC between the Swiss Institute of Bioinformatics and the EMBL outstation -
CC the European Bioinformatics Institute. There are no restrictions on its
CC use as long as its content is in no way modified and this statement is not
CC removed.
CC --------------------------------------------------------------------------
DR EMBL; AF059245; AAC14348.1; -. [EMBL / GenBank / DDBJ] [CoDingSequence]
DR EMBL; AF103946; AAD19577.1; -. [EMBL / GenBank / DDBJ] [CoDingSequence]
DR EMBL; AJ006757; CAA07225.1; -. [EMBL / GenBank / DDBJ] [CoDingSequence]
DR EMBL; AJ006756; CAA07224.1; -. [EMBL / GenBank / DDBJ] [CoDingSequence]
DR PIR; JE0280; JE0280.
DR HSSP; Q96J12; 1FM9. [HSSP ENTRY / SWISS-3DIMAGE / PDB]
DR SMR; O62807; 233-504.
DR InterPro; IPR000536; Hrmon_recept_lig.
DR InterPro; IPR001723; Stdhrmn_receptor.
DR InterPro; IPR008946; Str_ncl_receptor.
DR InterPro; IPR001628; Znf_C4steroid.
DR InterPro; Graphical view of domain structure.
DR Pfam; PF00104; Hormone_recep; 1.
DR Pfam; PF00105; zf-C4; 1.
DR Pfam; Graphical view of domain structure.
DR PRINTS; PR01288; PROXISOMEPAR.
DR PRINTS; PR01291; PROXISOMPAGR.
DR PRINTS; PR00398; STRDHORMONER.
DR PRINTS; PR00047; STROIDFINGER.
DR ProDom; PD000035; Znf_C4steroid; 1.
DR ProDom [Domain structure / List of seq. sharing at least 1 domain ]
DR SMART; SM00430; HOLI; 1.
DR SMART; SM00399; ZnF_C4; 1.
DR PROSITE; PS00031; NUCLEAR_REC_DBD_1; 1.
DR PROSITE; PS51030; NUCLEAR_REC_DBD_2; 1.
DR HOVERGEN [Family / Alignment / Tree]
DR BLOCKS; O62807.
DR ProtoNet; O62807.
DR ProtoMap; O62807.
DR PRESAGE; O62807.
DR DIP; O62807.
DR ModBase; O62807.
DR SWISS-2DPAGE; GET REGION ON 2D PAGE.
KW Activator; Alternative splicing; DNA-binding; Multigene family;
KW Nuclear protein; Phosphorylation; Receptor; Transcription;
KW Transcription regulation; Zinc-finger.
FT DNA_BIND 135 209 Nuclear receptor-type.
FT ZN_FING 138 158 C4-type.
FT ZN_FING 175 197 C4-type.
FT DOMAIN 317 504 Ligand-binding (Potential).
FT MOD_RES 111 111 Phosphoserine (by MAPK) (By similarity).
FT VARSPLIC 1 29 Missing (in isoform 1).
FT /FTId=VSP_003648.
FT CONFLICT 381 381 K -> R (in Ref. 3).
FT CONFLICT 426 426 G -> R (in Ref. 3).
SQ SEQUENCE 504 AA; 57512 MW; 57C0BDE901493D31 CRC64;
MGETLGDSLI DPESDAFDTL SANISQEVTM VDTEMPFWPT NFGISSVDLS VMDDHSHSFD
IKPFTTVDFS SISTPHYEDI PFPRADPMVA DYKYDLKLQD YQSAIKVEPV SPPYYSEKTQ
LYNKPHEEPS NSLMAIECRV CGDKASGFHY GVHACEGCKG FFRRTIRLKL IYDRCDLNCR
IHKKSRNKCQ YCRFQKCLAV GMSHNAIRFG RMPQAEKEKL LAEISSDIDQ LNPESADLRA
LAKHLYDSYI KSFPLTKAKA RAILTGKTTD KSPFVIYDMN SLMMGEDKIK FKHITPLQEQ
SKEVAIRIFQ GCQFRSVEAV QEITEYAKNI PGFVNLDLND QVTLLKYGVH EIIYTMLASL
MNKDGVLISE GQGFMTREFL KSLRKPFGDF MEPKFEFAVK FNALELDDSD LAIFIAVIIL
SGDRPGLLNV KPIEDIQDNL LQALELQLKL NHPESSQLFA KLLQKMTDLR QIVTEHVQLL
QVIKKTETDM SLHPLLQEIY KDLY
//