Swiss-Prot entry
ID NR4A1_RAT STANDARD; PRT; 597 AA.
AC P22829;
DT 01-AUG-1991 (Rel. 19, Created)
DT 01-OCT-1996 (Rel. 34, Last sequence update)
DT 01-MAY-2005 (Rel. 47, Last annotation update)
DE Orphan nuclear receptor NR4A1 (Orphan nuclear receptor HMR) (Nerve
DE growth factor induced protein I-B) (NGFI-B) (NUR77).
GN Name=Nr4a1; Synonyms=Hmr, Ngfib;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC Muridae; Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE.
RX MEDLINE=90166506; PubMed=3272167 [NCBI, ExPASy, EBI, Israel, Japan]; DOI=10.1016/0896-6273(88)90138-9;
RA Milbrandt J.;
RT "Nerve growth factor induces a gene homologous to the glucocorticoid
RT receptor gene.";
RL Neuron 1:183-188(1988).
RN [2]
RP CHARACTERIZATION.
RX MEDLINE=93361012; PubMed=8395013 [NCBI, ExPASy, EBI, Israel, Japan];
RA Wilson T.E., Fahrner T.J., Milbrandt J.;
RT "The orphan receptors NGFI-B and steroidogenic factor 1 establish
RT monomer binding as a third paradigm of nuclear receptor-DNA
RT interaction.";
RL Mol. Cell. Biol. 13:5794-5804(1993).
RN [3]
RP DNA-BINDING MOTIFS.
RX MEDLINE=92229411; PubMed=1314418 [NCBI, ExPASy, EBI, Israel, Japan];
RA Wilson T.E., Paulsen R.E., Padgett K.A., Milbrandt J.;
RT "Participation of non-zinc finger residues in DNA binding by two
RT nuclear orphan receptors.";
RL Science 256:107-110(1992).
RN [4]
RP PHOSPHORYLATION SITES SER-340 AND SER-350, AND MUTAGENESIS.
RX MEDLINE=94043340; PubMed=8227042 [NCBI, ExPASy, EBI, Israel, Japan];
RA Hirata Y., Kiuchi K., Chen H.-C., Milbrandt J., Guroff G.;
RT "The phosphorylation and DNA binding of the DNA-binding domain of the
RT orphan nuclear receptor NGFI-B.";
RL J. Biol. Chem. 268:24808-24812(1993).
RN [5]
RP X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 264-351 IN COMPLEX WITH NBRE,
RP AND METAL-BINDING.
RX MEDLINE=99260743; PubMed=10331876 [NCBI, ExPASy, EBI, Israel, Japan]; DOI=10.1038/8276;
RA Meinke G., Sigler P.B.;
RT "DNA-binding mechanism of the monomeric orphan nuclear receptor NGFI-
RT B.";
RL Nat. Struct. Biol. 6:471-477(1999).
CC -!- FUNCTION: Probable nuclear receptor. May act concomitantly with
CC NURR1 in regulating the expression of delayed-early genes during
CC liver regeneration. Binds the NGFI-B response element (NBRE) 5'-
CC AAAAGGTCA-3'.
CC -!- SUBUNIT: Binds DNA as a monomer.
CC -!- SUBCELLULAR LOCATION: Nuclear.
CC -!- TISSUE SPECIFICITY: Expressed in lung, brain and superior cervical
CC ganglia. High levels are seen in the adrenal tissue.
CC -!- INDUCTION: By nerve growth factor and during liver regeneration.
CC -!- PTM: Phosphorylation of Ser-350 results in decrease in NBRE
CC binding while phosphorylation of Ser-340 has little effect on it.
CC -!- SIMILARITY: Belongs to the nuclear hormone receptor family. NR4
CC subfamily.
CC -!- SIMILARITY: Contains 1 nuclear receptor DNA-binding domain.
CC --------------------------------------------------------------------------
CC This Swiss-Prot entry is copyright. It is produced through a collaboration
CC between the Swiss Institute of Bioinformatics and the EMBL outstation -
CC the European Bioinformatics Institute. There are no restrictions on its
CC use as long as its content is in no way modified and this statement is not
CC removed.
CC --------------------------------------------------------------------------
DR EMBL; U17254; AAA56770.1; ALT_INIT. [EMBL / GenBank / DDBJ] [CoDingSequence]
DR PDB; 1CIT; X-ray; A=-. [ExPASy / RCSB]
DR SMR; P22829; 264-352.
DR TRANSFAC; T00619; -.
DR Ensembl; ENSRNOG00000007607; Rattus_norvegicus
DR RGD; 620029; Nr4a1.
DR InterPro; IPR000536; Hrmon_recept_lig.
DR InterPro; IPR001723; Stdhrmn_receptor.
DR InterPro; IPR008946; Str_ncl_receptor.
DR InterPro; IPR001628; Znf_C4steroid.
DR InterPro; Graphical view of domain structure.
DR Pfam; PF00104; Hormone_recep; 1.
DR Pfam; PF00105; zf-C4; 1.
DR Pfam; Graphical view of domain structure.
DR PRINTS; PR01285; HMRNUCRECPTR.
DR PRINTS; PR01284; NUCLEARECPTR.
DR PRINTS; PR00398; STRDHORMONER.
DR PRINTS; PR00047; STROIDFINGER.
DR ProDom; PD000035; Znf_C4steroid; 1.
DR ProDom [Domain structure / List of seq. sharing at least 1 domain ]
DR SMART; SM00430; HOLI; 1.
DR SMART; SM00399; ZnF_C4; 1.
DR PROSITE; PS00031; NUCLEAR_REC_DBD_1; 1.
DR PROSITE; PS51030; NUCLEAR_REC_DBD_2; 1.
DR CMR; P22829.
DR HOVERGEN [Family / Alignment / Tree]
DR BLOCKS; P22829.
DR ProtoNet; P22829.
DR ProtoMap; P22829.
DR PRESAGE; P22829.
DR DIP; P22829.
DR ModBase; P22829.
DR SWISS-2DPAGE; GET REGION ON 2D PAGE.
KW 3D-structure; DNA-binding; Nuclear protein; Phosphorylation; Receptor;
KW Transcription; Transcription regulation; Zinc-finger.
FT DNA_BIND 263 338 Nuclear receptor-type.
FT ZN_FING 266 286 C4-type.
FT ZN_FING 302 326 C4-type.
FT DOMAIN 408 458 Ligand-binding (Potential).
FT DOMAIN 80 91 Poly-Ser.
FT DOMAIN 182 186 Poly-Pro.
FT DOMAIN 582 585 Poly-Pro.
FT MOD_RES 340 340 Phosphoserine (by PKA).
FT MOD_RES 350 350 Phosphoserine (by PKA).
FT MUTAGEN 340 340 S->A: Loss of phosphorylation.
FT MUTAGEN 350 350 S->A: Loss of phosphorylation.
FT MUTAGEN 345 345 R->K: Decreased NBRE binding.
FT MUTAGEN 348 348 L->V: Almost complete loss of NBRE
FT binding.
FT STRAND 275 277
FT TURN 278 279
FT STRAND 280 282
FT HELIX 284 296
FT TURN 297 297
FT TURN 312 317
FT HELIX 319 328
FT TURN 329 330
FT HELIX 333 335
FT HELIX 339 341
FT TURN 342 343
SQ SEQUENCE 597 AA; 64282 MW; 9CFA987112337E53 CRC64;
MPCIQAQYGT PATSPGPRDH LTGDPLALEF SKPTMDLASP ETAPTAPATL PSFSTFMDGG
YTGEFDTFLY QLPGTAQPCS SASSTSSSSS SATSPASASF KFEDFQVYGC YPGTLSGPLD
ETLSSSGSDY YGSPCSAPSP PTPNFQPSQL SPWDGSFGHF SPSQTYEGLR VWTEQLPKAS
GPPPPPTFFS FSPPTGPSPS LAQSSLKLFP APATHQLGEG ESYSVPAAFP GLAPTSPNCD
TSGILDAPVT STKARSGSSG GSEGRCAVCG DNASCQHYGV RTCEGCKGFF KRTVQKSAKY
ICLANKDCPV DKRRRNRCQF CRFQKCLAVG MVKEVVRTDS LKGRRGRLPS KPKQPPDASP
TNLLTSLIRA HLDSGPNTAK LDYSKFQELV LPRFGKEDAG DVQQFYDLLS GSLDVIRKWA
EKIPGFIELS PGDQDLLLES AFLELFILRL AYRSKPGEGK LIFCSGLVLH RLQCARGFGD
WIDNILAFSR SLHSLGVDVP AFACLSALVL ITDRHGLQDP RRVEELQNRI ASCLKEHMAA
VAGDPQPASC LSRLLGKLPE LRTLCTQGLQ RIFCLKLEDL VPPPPIVDKI FMDTLSF
//