Swiss-Prot entry

ID   GCR_SHEEP      STANDARD;      PRT;   314 AA.
AC   P35547;
DT   01-JUN-1994 (Rel. 29, Created)
DT   01-JUN-1994 (Rel. 29, Last sequence update)
DT   01-MAY-2005 (Rel. 47, Last annotation update)
DE   Glucocorticoid receptor (GR) (Fragment).
GN   Name=NR3C1; Synonyms=GRL;
OS   Ovis aries (Sheep).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; 
OC   Mammalia; Eutheria; Laurasiatheria; Cetartiodactyla; Ruminantia; 
OC   Pecora; Bovidae; Caprinae; Ovis. 
OX   NCBI_TaxID=9940;
RN   [1]
RP   NUCLEOTIDE SEQUENCE.
RC   TISSUE=Liver;
RX   MEDLINE=92384883; PubMed=1515021 [NCBI, ExPASy, EBI, Israel, Japan];
RA   Yang K., Hammond G.L., Challis J.R.;
RT   "Characterization of an ovine glucocorticoid receptor cDNA and
RT   developmental changes in its mRNA levels in the fetal sheep
RT   hypothalamus, pituitary gland and adrenal.";
RL   J. Mol. Endocrinol. 8:173-180(1992).
CC   -!- FUNCTION: Receptor for glucocorticoids (GC). Has a dual mode of
CC       action: as a transcription factor that binds to glucocorticoid
CC       response elements (GRE) and as a modulator of other transcription
CC       factors. Affects inflammatory responses, cellular proliferation
CC       and differentiation in target tissues (By similarity).
CC   -!- SUBUNIT: Heteromultimeric cytoplasmic complex with HSP90, HSP70,
CC       and an immunophilin. Upon ligand binding the complex undergoes a
CC       conformation change and moves to the nucleus, where it
CC       dissociates. Binds to DNA as a homodimer, and as heterodimer with
CC       NR3C2 or the retinoid X receptor. Interacts with the coactivator
CC       NCOA6, leading to a strong increase in transcription of target
CC       genes. Interaction with numerous other transcription factors
CC       modulates transcription activation. Interacts with TRIM28 (By
CC       similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasmic in the absence of ligand;
CC       nuclear after ligand-binding (By similarity).
CC   -!- DOMAIN: Composed of three domains: a modulating N-terminal domain,
CC       a DNA-binding domain and a C-terminal steroid-binding domain.
CC   -!- PTM: Increased proteasome-mediated degradation in response to
CC       glucocorticoids (By similarity).
CC   -!- PTM: Phosphorylated in the absence of hormone; becomes
CC       hyperphosphorylated in the presence of glucocorticoids (By
CC       similarity).
CC   -!- PTM: Sumoylated; this reduces transcription transactivation (By
CC       similarity).
CC   -!- SIMILARITY: Belongs to the nuclear hormone receptor family. NR3
CC       subfamily.
CC   -!- SIMILARITY: Contains 1 nuclear receptor DNA-binding domain.
CC   --------------------------------------------------------------------------
CC   This Swiss-Prot entry is copyright. It is produced through a collaboration
CC   between  the Swiss Institute of Bioinformatics  and the  EMBL outstation -
CC   the European Bioinformatics Institute.  There are no  restrictions on  its
CC   use as long as its content is in no way modified and this statement is not
CC   removed.
CC   --------------------------------------------------------------------------
DR   EMBL; X70407; CAA49851.1; -. [EMBL / GenBank / DDBJ] [CoDingSequence]
DR   EMBL; S44554; AAB23141.1; -. [EMBL / GenBank / DDBJ] [CoDingSequence]
DR   PIR; S31868; S31868.
DR   HSSP; P06536; 1GDC. [HSSP ENTRY / SWISS-3DIMAGE / PDB]
DR   TRANSFAC; T05075; -.
DR   InterPro; IPR001409; Glcrtcd_receptor.
DR   InterPro; IPR001628; Znf_C4steroid.
DR   InterPro; Graphical view of domain structure.
DR   Pfam; PF02155; GCR; 1.
DR   Pfam; PF00105; zf-C4; 1.
DR   Pfam; Graphical view of domain structure.
DR   PRINTS; PR00047; STROIDFINGER.
DR   ProDom; PD000035; Znf_C4steroid; 1.
DR   ProDom [Domain structure / List of seq. sharing at least 1 domain ]
DR   SMART; SM00399; ZnF_C4; 1.
DR   PROSITE; PS00031; NUCLEAR_REC_DBD_1; 1.
DR   PROSITE; PS51030; NUCLEAR_REC_DBD_2; 1.
DR   HOVERGEN [Family / Alignment / Tree]
DR   BLOCKS; P35547.
DR   ProtoNet; P35547.
DR   ProtoMap; P35547.
DR   PRESAGE; P35547.
DR   DIP; P35547.
DR   ModBase; P35547.
DR   SWISS-2DPAGE; GET REGION ON 2D PAGE.
KW   DNA-binding; Nuclear protein; Receptor; Steroid-binding;
KW   Trans-acting factor; Transcription; Transcription regulation;
KW   Ubl conjugation; Zinc-finger.
FT   NON_TER       1      1
FT   DOMAIN       <1    281       Modulating.
FT   DOMAIN      261    280       Glu/Ser/Pro/Thr-rich (PEST region)
FT                                (Potential).
FT   DNA_BIND    282   >314       Nuclear receptor-type.
FT   ZN_FING     282   >314       C4-type.
FT   NON_TER     314    314
SQ   SEQUENCE   314 AA;  33562 MW;  5899DCB75B79CAD8 CRC64;
     ASAAVSAAPT EKEFPKTHSD VSSEQQNLKG QKGSNGGSMK LHTTDQSTFD IWRKKLQDLE
     FSSESPSKET SDSPWRSDIL IDENCLLSPL AGEDDSFLLE GSSNEDCKPL LLPDAKPKIK
     DNGDLILPSP NSVPLPQVKT EKEDFIELCT PGVIKQEKLG PVYCQASFPG ANIIGNKMSA
     ISVHGVSTSG GQMYHYDMNT ASLSQQQDQK PIFKVIPPIP VGSENWNRCQ GSGDDSLTSL
     GTLNFSGRSV FSNGYSSPGM RPDVSSPPSS SSAATGPPPK LCLVCSDEAS GCHYGVLTCG
     SCKVFFKRAV EGQH
//