Swiss-Prot entry
ID GCR_RABIT STANDARD; PRT; 772 AA.
AC P59667;
DT 10-OCT-2003 (Rel. 42, Created)
DT 10-OCT-2003 (Rel. 42, Last sequence update)
DT 01-MAY-2005 (Rel. 47, Last annotation update)
DE Glucocorticoid receptor (GR).
GN Name=NR3C1; Synonyms=GRL;
OS Oryctolagus cuniculus (Rabbit).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Glires; Lagomorpha; Leporidae;
OC Oryctolagus.
OX NCBI_TaxID=9986;
RN [1]
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Lens epithelium;
RA James E.R., Robertson L.L.;
RT "Rabbit glucocorticoid receptor from lens epithelial cells.";
RL Submitted (OCT-2002) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Receptor for glucocorticoids (GC). Has a dual mode of
CC action: as a transcription factor that binds to glucocorticoid
CC response elements (GRE) and as a modulator of other transcription
CC factors. Affects inflammatory responses, cellular proliferation
CC and differentiation in target tissues (By similarity).
CC -!- SUBUNIT: Heteromultimeric cytoplasmic complex with HSP90, HSP70,
CC and an immunophilin. Upon ligand binding the complex undergoes a
CC conformation change and moves to the nucleus, where it
CC dissociates. Binds to DNA as a homodimer, and as heterodimer with
CC NR3C2 or the retinoid X receptor. Interacts with the coactivator
CC NCOA6, leading to a strong increase in transcription of target
CC genes. Interaction with numerous other transcription factors
CC modulates transcription activation. Interacts with TRIM28 (By
CC similarity).
CC -!- SUBCELLULAR LOCATION: Cytoplasmic in the absence of ligand;
CC nuclear after ligand-binding (By similarity).
CC -!- DOMAIN: Composed of three domains: a modulating N-terminal domain,
CC a DNA-binding domain and a C-terminal steroid-binding domain.
CC -!- PTM: Increased proteasome-mediated degradation in response to
CC glucocorticoids (By similarity).
CC -!- PTM: Phosphorylated in the absence of hormone; becomes
CC hyperphosphorylated in the presence of glucocorticoids (By
CC similarity).
CC -!- PTM: Sumoylated; this reduces transcription transactivation (By
CC similarity).
CC -!- SIMILARITY: Belongs to the nuclear hormone receptor family. NR3
CC subfamily.
CC -!- SIMILARITY: Contains 1 nuclear receptor DNA-binding domain.
CC --------------------------------------------------------------------------
CC This Swiss-Prot entry is copyright. It is produced through a collaboration
CC between the Swiss Institute of Bioinformatics and the EMBL outstation -
CC the European Bioinformatics Institute. There are no restrictions on its
CC use as long as its content is in no way modified and this statement is not
CC removed.
CC --------------------------------------------------------------------------
DR EMBL; AY161275; AAN75442.1; -. [EMBL / GenBank / DDBJ] [CoDingSequence]
DR HSSP; P06536; 1GDC. [HSSP ENTRY / SWISS-3DIMAGE / PDB]
DR SMR; P59667; 412-490, 518-772.
DR InterPro; IPR001409; Glcrtcd_receptor.
DR InterPro; IPR000536; Hrmon_recept_lig.
DR InterPro; IPR001723; Stdhrmn_receptor.
DR InterPro; IPR008946; Str_ncl_receptor.
DR InterPro; IPR001628; Znf_C4steroid.
DR InterPro; Graphical view of domain structure.
DR Pfam; PF02155; GCR; 1.
DR Pfam; PF00104; Hormone_recep; 1.
DR Pfam; PF00105; zf-C4; 1.
DR Pfam; Graphical view of domain structure.
DR PRINTS; PR00528; GLCORTICOIDR.
DR PRINTS; PR00398; STRDHORMONER.
DR PRINTS; PR00047; STROIDFINGER.
DR ProDom; PD000035; Znf_C4steroid; 1.
DR ProDom [Domain structure / List of seq. sharing at least 1 domain ]
DR SMART; SM00430; HOLI; 1.
DR SMART; SM00399; ZnF_C4; 1.
DR PROSITE; PS00031; NUCLEAR_REC_DBD_1; 1.
DR PROSITE; PS51030; NUCLEAR_REC_DBD_2; 1.
DR HOVERGEN [Family / Alignment / Tree]
DR BLOCKS; P59667.
DR ProtoNet; P59667.
DR ProtoMap; P59667.
DR PRESAGE; P59667.
DR DIP; P59667.
DR ModBase; P59667.
DR SWISS-2DPAGE; GET REGION ON 2D PAGE.
KW DNA-binding; Nuclear protein; Phosphorylation; Receptor;
KW Steroid-binding; Trans-acting factor; Transcription;
KW Transcription regulation; Ubl conjugation; Zinc-finger.
FT DOMAIN 1 415 Modulating.
FT DOMAIN 395 414 Glu/Ser/Pro/Thr-rich (PEST region)
FT (Potential).
FT DNA_BIND 416 481 Nuclear receptor-type.
FT ZN_FING 416 436 C4-type.
FT ZN_FING 452 476 C4-type.
FT DOMAIN 482 522 Hinge.
FT DOMAIN 528 772 Steroid-binding.
FT MOD_RES 113 113 Phosphoserine (By similarity).
FT MOD_RES 203 203 Phosphoserine (By similarity).
FT MOD_RES 211 211 Phosphoserine (By similarity).
FT MOD_RES 226 226 Phosphoserine (By similarity).
SQ SEQUENCE 772 AA; 84863 MW; C16D6CF799F83148 CRC64;
MDSKESLSPP GREEVPSSVL RPAERGNVMD LYKTLRGGAP VRVPASSPSL APAAQPDSKQ
QRLAVDFPKG SASNAQQPDL SRAVSLSMGL YMGETETKVM GSDLAFPQQG QTSLSSGETD
FRLLEESIAS LNRSASGADN PRSTAPAAGS AAPTEGFPKT HSDLASERQN PKGQTGGSAG
SAKLHPTDQS TFDILQDLEF SGSPSKDRSE SPWRSDLLMD ENCLLSPLAG EDDPFLLEGN
SSEDCKPLIL PDTKPKIKDN GDLILSNSNN VPLPQVKTEK EDFIELCTPG VIKQEKLGPV
YCQASFSGAN IIGNKISAIS VHGVSTSGGQ MYHYDMNAQQ QEQKPLFNVI PPIPVGSENW
NRCQGSGDDN LTSLGTMNFP GRSVFSNGYS SPGMRPDVSS PPSNSTTAAG PPPKLCLVCS
DEASGCHYGV LTCGSCKVFF KRAVKGQHNY LCAGRNDCII DKIRRKNCPA CRYRKCLQAG
MNLEARKTKK KIKGIQQTST GVSQETSENP SNRTVVPAAL PQLTPTLVSL LEVIEPEVLY
AGYDSSVPDS TWRIMTTLNM LGGRQVIAAV KWAKAIPGFR NLHLDDQMTL LQYSWMFLMA
FALGWRSYKQ SSGNMLCFAP DLVINEQRMT LPYMYDQCKH MLFVSSELKR LQVSYEEYLC
MKTLLLLSTV PKEGLKSQEL FDEIRMTYIK ELGKAIVKRE GNSSQNWQRF YQLTKLLDSM
HEVVENLLHY CFQTFLDKTM SIEFPEMLAE IITNQIPKYS NGNIKKLLFH QK
//