NucleaRDB: Extraction of mutation data from the literature

2005, NucleaRDB.

This data was extracted from Medline abstracts and full texts (when available) in an automated manner.

The table below describes different point mutations at a given position and provides links to other documents. The sentence(s) where the point mutations in VDR_MOUSE at position 380 were found are listed after the table.

Point mutations at position Q380 in VDR_MOUSE

Protein	VDR_MOUSE (P48281) Gene: Vdr,Nr1i (other point mutations)	Swiss-Prot Cross-reference table Family page
Position	Q380
General numbering (NucleaRDB)	1045
Domain	LBD HELIX 10-11
Family alignments	1I1 Vitamin D3 (VDR) 1I Vitamin D3-like (VDR,PXR,CAR) 1 Thyroid hormone like (TR,RAR,ROR,PPAR,VDR
Other point mutations at the same position	Position 385 in 1I1 Vitamin D3 (VDR) family Position 380 in 1I Vitamin D3-like (VDR,PXR,CAR) family Position 369 in 1 Thyroid hormone like (TR,RAR,ROR,PPAR,VDR family
Reference #1	Barry JB, Leong GM, Church WB, Issa LL, Eisman JA, Gardiner EM J Biol Chem 2003 Mar 7;278(10):8224-8.	Medline
Text source	HTML and PDF full texts
Point mutation	Q380A (True positive)
Point mutation	Q380E (True positive)

Relevant sentences

Reference #1 (Barry JB et al.): Q380A

Mutant Delta 389 was further mutated to create Delta 389Q380E , Delta 389D384K , Delta 389R386E , and Delta 389AX3 , the last being a triple point mutant with coordinate Q380A , D384A , and R386A changes
Mutant 389 was further mutated to create 389Q380E , 389D384K , 389R386E , and 389AX3 , the last being a triple point mutant with coordinate Q380A , D384A , and R386A changes

Reference #1 (Barry JB et al.): Q380E

Mutant Delta 389 was further mutated to create Delta 389Q380E , Delta 389D384K , Delta 389R386E , and Delta 389AX3 , the last being a triple point mutant with coordinate Q380A , D384A , and R386A changes
Yeast two-hybrid assay of strains expressing HA-tagged SKIP (amino acids 145-536) , with mVDR wild type and mutants: A , Delta 397 , Delta 389 , or Delta 380 ; B , Delta 389 , Delta 389Q380E , Delta 389D384K , Delta 389R386E , and Delta 389AX3 ; C , AX3 and treated with vehicle (open bars) or 10-8 M 1 , 25(OH)2D3 (solid bars)
To investigate their potential involvement in SKIP / NCoA-62 interaction , these three residues were individually mutated to retain similar bulk but altered charge in the context of Delta 389 (Q380E , D384K , or R386E ; Fig. 1 A)
Mutant 389 was further mutated to create 389Q380E , 389D384K , 389R386E , and 389AX3 , the last being a triple point mutant with coordinate Q380A , D384A , and R386A changes
To investigate their potential involvement in SKIP / NCoA-62 interaction , these three residues were individually mutated to retain similar bulk but altered charge in the context of 389 (Q380E , D384K , or R386E ; Fig. 1A)
Yeast two-hybrid assay of strains expressing HA-tagged SKIP (amino acids 145536) , with mVDR wild type and mutants: A , 397 , 389 , or 380 ; B , 389 , 389Q380E , 389D384K , 389R386E , and 389AX3 ; C , AX3 and treated with vehicle (open bars) or 10 8 M 1 , 25(OH)2D3 (solid bars)

F.Horn (nucleardbcmbi.kun.nl), 21-Apr-2005