NucleaRDB: Extraction of mutation data from the literature

2005, NucleaRDB.

This data was extracted from Medline abstracts and full texts (when available) in an automated manner.

The table below describes different point mutations at a given position and provides links to other documents. The sentence(s) where the point mutations in ANDR_HUMAN at position 94 were found are listed after the table.

Point mutations at position S94 in ANDR_HUMAN

Protein	ANDR_HUMAN (P10275) Gene: AR,DHTR, NR3C (other point mutations)	Swiss-Prot Cross-reference table Family page
Position	S94
General numbering (NucleaRDB)	-
Domain	N-term
Family alignments	3C4 Androgen (AR) 3C Glucocorticoid-like (GR,MR,PR,AR) 3 Estrogen like (ER,ERR,GR,MR,PR,AR)
Other point mutations at the same position	Position 75 in 3C4 Androgen (AR) family Position 39 in 3C Glucocorticoid-like (GR,MR,PR,AR) family Position 1 in 3 Estrogen like (ER,ERR,GR,MR,PR,AR) family
Reference #1	Gioeli D, Ficarro SB, Kwiek JJ, Aaronson D, Hancock M, Catling AD, White FM, Christian RE, Settlage RE, Shabanowitz J, Hunt DF, Weber MJ J Biol Chem 2002 Aug 9;277(32):29304-14.	Medline
Text source	HTML and PDF full texts
Point mutation	S94A (True positive)
Cited point mutation	Ser94Ala,Ser-94
Reference #2	Fu M, Rao M, Wu K, Wang C, Zhang X, Hessien M, Yeung YG, Gioeli D, Weber MJ, Pestell RG J Biol Chem 2004 Jul 9;279(28):29436-49. Epub 2004 Apr 30.	Medline
Text source	HTML full text
Point mutation	S94A (Not yet checked)

Relevant sentences

Reference #2 (Fu M et al.): S94A

The ARwt and point mutants of the AR phosphorylation site (AR S81A , AR S94A , AR S256A , AR S308A , AR S426A , and AR S650A) were described elsewhere (23 )
Regulation of AR signaling was selectively reduced by the S94A mutant
The S94A mutant was significantly reduced in trans-activation by p300 (Fig. 9D )

Reference #1 (Gioeli D et al.): Ser-94

Ser-94 appears constitutively phosphorylated
Phosphopeptides 2 and 3 were lost when Ser-94 was mutated to Ala ; phosphopeptide 2 also had a cycle release at position 11 , consistent with Ser-94 phosphorylation
Phosphopeptide 3 most likely represents a partial tryptic digest of a Ser-94 containing phosphopeptide
The exceptions are Ser-94 , which did not change in response to hormone , and Ser-81 whose phosphorylation was still increasing at 6 h
Phosphopeptides 2 and -->were lost when Ser-94 was mutated to Ala ; phosphopeptide 2 also had a cycle release at position 11 , consistent with Ser-94 phosphorylation

F.Horn (nucleardbcmbi.kun.nl), 21-Apr-2005