The pairwise alignment has been generated with the "genewise" program of the Wise2 package. The output shows the protein sequence on the first line, followed by a line indicating the similarity level of the match, followed by 4 lines representing the DNA sequence; The DNA sequence descending in triplets, each triplet being a codon. The translation of each codon is shown above it.
Between the two protein sequences a line indicating the similarity of the match is printed. "+" indicates a conservative mismatch while "- "indicates a dramatic one. Mismatches are indicated by "***" in the left column in addition to "!" at the relevant position(s).
genewise $Name: wise2-2-0 $ (unreleased release)
This program is freely distributed under a GPL. See source directory
Copyright (c) GRL limited: portions of the code are from separate copyright
Query protein: KCNKH_HUMAN
Comp Matrix: blosum62.bla
Gap open: 12
Gap extension: 2
Start/End default
Target Sequence AF339912_2
Strand: forward
Start/End (protein) default
Gene Paras: human.gf
Codon Table: codon.table
Subs error: 1e-05
Indel error: 1e-05
Model splice? model
Model codon bias? flat
Model intron bias? tied
Null model syn
Algorithm 623
genewise output
Score 822.68 bits over entire alignment
Scores as bits over a synchronous coding model
Warning: The bits scores is not probablistically correct for single seqs
See WWW help for more info.
Warning: mismatch(es) found
KCNKH_HUMAN 1 MYRPRARAAPEGRVRGCAVPSTVLLLLAYLAYLALGTGVFWTLEGRAAQ
MYRPRARAAPEGRVRGCAVP-TVLLLLAYLAYLALGTGVFW+LEGRAAQ
MYRPRARAAPEGRVRGCAVPGTVLLLLAYLAYLALGTGVFWxLEGRAAQ
AF339912_2 1 atcccgcggcggagcgtggcgagccccgtcgtcgcgaggttacggcggc
tagcgcgcccaggtgggctcgctttttcatcatctgcgttgctaggcca
gcagacggtcgcgcgccggcccgcggcccgtcgggcccgcgNggccggg
*** ! !
KCNKH_HUMAN 50 DSSRSFQRDKWELLQNFTCLDRPALDSLIRDVVQAYKNGASLLSNTTSM
DSSRSFQRDKWELLQNFTCLDRPALDSLIRDVVQAYKNGASLLSNTTSM
DSSRSFQRDKWELLQNFTCLDRPALDSLIRDVVQAYKNGASLLSNTTSM
AF339912_2 148 gtacatccgatgctcatatcgccgcgtcacgggcgtaaggaccaaaaaa
acgggtagaagattaatcgtagcctacttgattacaaagcgttgaccgt
ccccccgccggggggccgtgccgggcggcgtccaacacacccccccccg
KCNKH_HUMAN 99 GRWELVGSFFFSVSTITTIGYGNLSPNTMAARLFCIFFALVGIPLNLVV
GRWELVGSFFFSVSTITTIGYGNLSPNTMAARLFCIFFALVGIPLNLVV
GRWELVGSFFFSVSTITTIGYGNLSPNTMAARLFCIFFALVGIPLNLVV
AF339912_2 295 gctgcggtttttgtaaaaagtgacacaaaggccttattgcggaccacgg
gggattgctttctcctcctgagatgcactccgttgtttcttgtctattt
gcggcgccccttgccccctctccgcccggtccccccctctggcacccgg
KCNKH_HUMAN 148 LNRLGHLMQQGVNHWASRLGGTWQDPDKARWLAGSGALLSGLLLFLLLP
LNRLGHLMQQGVNHWASRLGGTWQDPDKARWLAGSGALLSGLLLFLLLP
LNRLGHLMQQGVNHWASRLGGTWQDPDKARWLAGSGALLSGLLLFLLLP
AF339912_2 442 caccgccaccggactgaacggatcgcgagctcggtggcctgccctcccc
tagtgattaagtaagcggtggcgaacaacggtcgcgcttcgtttttttc
ccaggtcgggaaccgccgggccggttcggggggctccccgccgccggga
KCNKH_HUMAN 197 PLLFSHMEGWSYTEGFYFAFITLSTVGFGDYVIGMNPSQRYPLWYKNMV
PLLFSHMEGWSYTEGFYFAFITLSTVGFGDYVIGMNPSQRYPLWYKNMV
PLLFSHMEGWSYTEGFYFAFITLSTVGFGDYVIGMNPSQRYPLWYKNMV
AF339912_2 589 cccttcaggtataggtttgtaacaaggtggtgagaactcatccttaaag
ctttcatagggacagtatcttctgctgtgaattgtaccagactgaaatt
ggccccggcgccagcccccccccccgcccccgtagcccggcaggcgcgg
KCNKH_HUMAN 246 SLWILFGMAWLALIIKLILSQLETPGRVCSCCHHSSKEDFKSQSWRQGP
SLWILFGMAWLALI-KLILSQLETPGRVCSCCHHSSKEDFKSQSWRQGP
SLWILFGMAWLALI!KLILSQLETPGRVCSCCHHSSKEDFKSQSWRQGP
AF339912_2 736 tctactgagtcgta2acactccgacgagttttccataggtatcatacgc
ctgtttgtcgtctt atttcataccggtgcggaagcaaatacagggagc
cggcctggaggcgc accccggggaggatcccccctgaccgcacgagat
*** !
KCNKH_HUMAN 295 DREPESHSPQQGCYPEGPMGIIQHLEPSAHAAGCGKDS
DREPESHSPQQGCYPEGPMGIIQHLEPSAHAAGCGKDS
DREPESHSPQQGCYPEGPMGIIQHLEPSAHAAGCGKDS
AF339912_2 882 gcgcgtctcccgttcggcagaacccgctgcgggtgaga
agacacaccaaggacagctgttaatacccaccgggaag
cggagcccagaactagacgacagtgatttctactcgcc
//
cmbi.kun.nl), 28-Jun-2005