The pairwise alignment has been generated with the "genewise" program of the Wise2 package. The output shows the protein sequence on the first line, followed by a line indicating the similarity level of the match, followed by 4 lines representing the DNA sequence; The DNA sequence descending in triplets, each triplet being a codon. The translation of each codon is shown above it.
Between the two protein sequences a line indicating the similarity of the match is printed. "+" indicates a conservative mismatch while "- "indicates a dramatic one. Mismatches are indicated by "***" in the left column in addition to "!" at the relevant position(s).
genewise $Name: wise2-2-0 $ (unreleased release)
This program is freely distributed under a GPL. See source directory
Copyright (c) GRL limited: portions of the code are from separate copyright
Query protein: KCNKF_RAT
Comp Matrix: blosum62.bla
Gap open: 12
Gap extension: 2
Start/End default
Target Sequence AF294353_2
Strand: forward
Start/End (protein) default
Gene Paras: human.gf
Codon Table: codon.table
Subs error: 1e-05
Indel error: 1e-05
Model splice? model
Model codon bias? flat
Model intron bias? tied
Null model syn
Algorithm 623
genewise output
Score 581.48 bits over entire alignment
Scores as bits over a synchronous coding model
Warning: The bits scores is not probablistically correct for single seqs
See WWW help for more info.
Warning: mismatch(es) found
KCNKF_RAT 82 GSFYFAITVITTIGYGHAAPGTDSGKVFCMFYALLGIPLTLVTFQSLGE
GSFYFAITVITTIGYGHAAPGTDSGKVFCMFYALLGIPLTLVTFQSLGE
GSFYFAITVITTIGYGHAAPGTDSGKVFCMFYALLGIPLTLVTFQSLGE
AF294353_2 3 gttttgaagaaaagtgcggcgagtgagttattgccgaccacgatcacgg
gctatctcttcctgagacccgcacgattgttacttgtctcttctagtga
cccccccgcccccgtctttacccgcagccgctccgcccgtgtccgcgcg
KCNKF_RAT 131 RLNALVRCLLLAAKRCLGLRRPHVSAENMVVAGLLLCAATLALGAAAFA
RLNALVRCLLLAAKRCLGLRRPHVSAENMVVAGLLLCAATLALGAAAFA
RLNALVRCLLLAAKRCLGLRRPHVSAENMVVAGLLLCAATLALGAAAFA
AF294353_2 150 ccagcgctcccggactcgcccccgtggaaggggtcctggacgcggggtg
gtacttggtttccaggtgtggcatccaatttcgtttgccctctgccctc
tgcggagtggaacgccgcgggacgttgcgggtggggccccgcgcctccg
KCNKF_RAT 180 HFEGWTFFHAYYYCFITLTTIGFGDFVALQRDEALQKKPPYVAFSFLYI
HFEGWTFFHAYYYCFITLTTIGFGDFVALQRDEALQKKPPYVAFSFLYI
HFEGWTFFHAYYYCFITLTTIGFGDFVALQRDEALQKKPPYVAFSFLYI
AF294353_2 297 ctggtattcgtttttaacaaagtggtggtcagggccaacctggtatcta
ataggcttacaaagttctcctgtgattctagaactaaaccatctgttat
ccgcgccccccccccccccccccaccggggacgggggggccaccccccc
KCNKF_RAT 229 LLGLTVIGAFLNLVVLRFLASAEAPERAALRRASVFRRGAPESRVRIPY
LLGLTVIGAFLNLVVLRFLASAEAPERAALRRASVFRRGAPESRVRIPY
LLGLTVIGAFLNLVVLRFLASAEAPERAALRRASVFRRGAPESRVRIPY
AF294353_2 444 ccgcagaggtcacggcctcgagggcgcggcccgagtcaggcgacgcact
ttgtcttgcttattttgttcgcaccagcctggcgttgggccaggtgtca
gggcactttcccggcgactcctgcagcatccttcgccgggcgccccctc
KCNKF_RAT 278 PFHPLETWARDNPAFSPPLSPEAVHDCHSSPDRLRARRKSI
PFHPLETWARDNPAFSPPLSPEAVHDCHSSPDRLRAR-KSI
PFHPLETWARDNPAFSPPLSPEAVHDCHSSPDRLRARWKSI
AF294353_2 591 ctcccgatgcgacgttcctacgggcgtcaacgaccgctata
ctactacgcgaacctccctgcactaagaggcagtgcggact
cttgggcgctccgccactgcaaagttccccacacgcgggcc
*** !
//
cmbi.kun.nl), 28-Jun-2005