Swiss-Prot entry
ID KCMB4_RAT STANDARD; PRT; 210 AA.
AC Q9ESK8;
DT 05-JUL-2004 (Rel. 44, Created)
DT 05-JUL-2004 (Rel. 44, Last sequence update)
DT 10-MAY-2005 (Rel. 47, Last annotation update)
DE Calcium-activated potassium channel beta subunit 4 (Calcium-activated
DE potassium channel, subfamily M, beta subunit 4) (Maxi K channel beta
DE subunit 4) (BK channel beta subunit 4) (Slo-beta 4) (K(VCA)beta 4)
DE (Charybdotoxin receptor beta subunit 4) (BKbeta4).
GN Name=Kcnmb4;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC Muridae; Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Brain;
RA Ohya S., Ohi Y., Imaizumi Y.;
RT "rat calcium activated potassium channel beta 4 subunit (KCNMB4).";
RL Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE.
RA Ha T.S., Park C.-S.;
RT "Molecular cloning of large-conductance calcium-activated potassium
RT channel beta 4 subunit from rat brain.";
RL Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Regulatory subunit of the calcium activated potassium
CC KCNMA1 (maxiK) channel. Modulates the calcium sensitivity and
CC gating kinetics of KCNMA1, thereby contributing to KCNMA1 channel
CC diversity. Decreases the gating kinetics and calcium sensitivity
CC of the KCNMA1 channel, but with fast deactivation kinetics. May
CC decrease KCNMA1 channel openings at low calcium concentrations but
CC increases channel openings at high calcium concentrations. Makes
CC KCNMA1 channel resistant to 100 nM charybdotoxin (CTX) toxin
CC concentrations (By similarity).
CC -!- SUBUNIT: Interacts with KCNMA1 tetramer. There are probably 4
CC molecules of KCMNB4 per KCNMA1 tetramer (By similarity).
CC -!- SUBCELLULAR LOCATION: Integral membrane protein (By similarity).
CC -!- DOMAIN: Resistance to charybdotoxin (CTX) toxin is mediated by the
CC extracellular domain (By similarity).
CC -!- PTM: Phosphorylated. Phosphorylation modulates its effect on
CC KCNMA1 activation kinetics (By similarity).
CC -!- PTM: N-glycosylated. A highly glycosylated form is promoted by
CC KCNMA1. Glycosylation, which is not required for the interaction
CC with KCNMA1 and subcellular location, increases protection against
CC charybdotoxin (By similarity).
CC -!- SIMILARITY: Belongs to the KCNMB family.
CC --------------------------------------------------------------------------
CC This Swiss-Prot entry is copyright. It is produced through a collaboration
CC between the Swiss Institute of Bioinformatics and the EMBL outstation -
CC the European Bioinformatics Institute. There are no restrictions on its
CC use as long as its content is in no way modified and this statement is not
CC removed.
CC --------------------------------------------------------------------------
DR EMBL; AB050637; BAB17595.1; -; mRNA. [EMBL / GenBank / DDBJ] [CoDingSequence]
DR EMBL; AY028605; AAK21964.1; -; mRNA. [EMBL / GenBank / DDBJ] [CoDingSequence]
DR Ensembl; ENSRNOG00000022297; Rattus_norvegicus
DR RGD; 620728; Kcnmb4.
DR GO; GO:0008076; C:voltage-gated potassium channel complex; ISS.
DR GO; GO:0015269; F:calcium-activated potassium channel activity; ISS.
DR GO; GO:0005515; F:protein binding; ISS.
DR GO; GO:0005513; P:calcium ion sensing; ISS.
DR GO; GO:0019228; P:generation of action potential; ISS.
DR GO; GO:0006813; P:potassium ion transport; ISS.
DR GO; GO:0001508; P:regulation of action potential; ISS.
DR GO; GO:0046928; P:regulation of neurotransmitter secretion; ISS.
DR GO; GO:0019229; P:regulation of vasoconstriction; ISS.
DR InterPro; IPR003930; BK_channel_beta.
DR InterPro; Graphical view of domain structure.
DR PANTHER; PTHR10258; BK_channel_beta; 1.
DR Pfam; PF03185; CaKB; 1.
DR Pfam; Graphical view of domain structure.
DR CMR; Q9ESK8.
DR ProDom [Domain structure / List of seq. sharing at least 1 domain]
DR HOVERGEN [Family / Alignment / Tree]
DR BLOCKS; Q9ESK8.
DR ProtoNet; Q9ESK8.
DR ProtoMap; Q9ESK8.
DR PRESAGE; Q9ESK8.
DR DIP; Q9ESK8.
DR ModBase; Q9ESK8.
DR SWISS-2DPAGE; GET REGION ON 2D PAGE.
KW Glycoprotein; Ion transport; Ionic channel; Phosphorylation;
KW Transmembrane; Transport.
FT TOPO_DOM 1 19 Cytoplasmic (Potential).
FT TRANSMEM 20 40 1 (Potential).
FT TOPO_DOM 41 167 Extracellular (Potential).
FT TRANSMEM 168 188 2 (Potential).
FT TOPO_DOM 189 210 Cytoplasmic (Potential).
FT CARBOHYD 53 53 N-linked (GlcNAc...) (By similarity).
FT CARBOHYD 90 90 N-linked (GlcNAc...) (By similarity).
SQ SEQUENCE 210 AA; 23885 MW; 11D49F58F9C6F1D4 CRC64;
MAKLRVSYEY TEAEDKSIRL GLFLIVSGIL SLFIFGFCWL SPALQDLQAT AANCTVLSVQ
QIGEVFECTF TCGTDCRGTS QYPCVQVYVN NSESNSRALL HSDQHQLLTN PKCSYIPPCK
RENQKNSESV MNWQQYWKDE IGSQPFTCYF NQHQRPEDVL LQRTHDEIVL LHCFLWPVVA
FVVGVLIVVL TICAKSLAVK AEAMKKRKFS
//