Swiss-Prot entry
ID KCMB1_HUMAN STANDARD; PRT; 190 AA.
AC Q16558; O00707; O00708; P78475; Q8TAX3; Q93005;
DT 01-NOV-1997 (Rel. 35, Created)
DT 01-NOV-1997 (Rel. 35, Last sequence update)
DT 10-MAY-2005 (Rel. 47, Last annotation update)
DE Calcium-activated potassium channel beta subunit 1 (Calcium-activated
DE potassium channel, subfamily M, beta subunit 1) (Maxi K channel beta
DE subunit 1) (BK channel beta subunit 1) (Slo-beta 1) (K(VCA)beta 1)
DE (Charybdotoxin receptor beta subunit 1) (BKbeta1) (Hbeta1) (Calcium-
DE activated potassium channel beta-subunit) (BKbeta) (Slo-beta).
GN Name=KCNMB1;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE (ISOFORM 1).
RC TISSUE=Uterus;
RX MEDLINE=96196569; PubMed=8612769 [NCBI, ExPASy, EBI, Israel, Japan]; DOI=10.1016/0014-5793(96)00151-2;
RA Meera P., Wallner M., Jiang Z., Toro L.;
RT "A calcium switch for the functional coupling between alpha (hslo) and
RT beta subunits (KV,Ca beta) of maxi K channels.";
RL FEBS Lett. 382:84-88(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE (ISOFORM 1).
RC TISSUE=Uterus;
RX MEDLINE=96335638; PubMed=8764643 [NCBI, ExPASy, EBI, Israel, Japan];
RA Dworetzky S.I., Boissard C.G., Lum-Ragan J.T., McKay M.C.,
RA Post-Munson D.J., Trojnacki J.T., Chang C.P., Gribkoff V.K.;
RT "Phenotypic alteration of a human BK (hSlo) channel by hSlobeta
RT subunit coexpression: changes in blocker sensitivity,
RT activation/relaxation and inactivation kinetics, and protein kinase A
RT modulation.";
RL J. Neurosci. 16:4543-4550(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE (ISOFORM 1).
RC TISSUE=Brain;
RX MEDLINE=96392390; PubMed=8799178 [NCBI, ExPASy, EBI, Israel, Japan]; DOI=10.1073/pnas.93.17.9200;
RA Tseng-Crank J., Godinot N., Johansen T.E., Ahring P.K., Strobaek D.,
RA Mertz R., Foster C.D., Olesen S.P., Reinhart P.H.;
RT "Cloning, expression, and distribution of a Ca(2+)-activated K+
RT channel beta-subunit from human brain.";
RL Proc. Natl. Acad. Sci. U.S.A. 93:9200-9205(1996).
RN [4]
RP NUCLEOTIDE SEQUENCE (ISOFORM 1).
RC TISSUE=Aortic smooth muscle;
RA Folander K., Biazzo D., Swanson R.;
RL Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE (ISOFORM 1).
RC TISSUE=Myometrium;
RX PubMed=12434576 [NCBI, ExPASy, EBI, Israel, Japan];
RA Mazzone J.N., Kaiser R.A., Buxton I.L.O.;
RT "Calcium-activated potassium channel expression in human myometrium:
RT effect of pregnancy.";
RL Proc. West. Pharmacol. Soc. 45:184-186(2002).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Pancreas;
RX MEDLINE=22388257; PubMed=12477932 [NCBI, ExPASy, EBI, Israel, Japan]; DOI=10.1073/pnas.242603899;
RA Strausberg R.L., Feingold E.A., Grouse L.H., Derge J.G.,
RA Klausner R.D., Collins F.S., Wagner L., Shenmen C.M., Schuler G.D.,
RA Altschul S.F., Zeeberg B., Buetow K.H., Schaefer C.F., Bhat N.K.,
RA Hopkins R.F., Jordan H., Moore T., Max S.I., Wang J., Hsieh F.,
RA Diatchenko L., Marusina K., Farmer A.A., Rubin G.M., Hong L.,
RA Stapleton M., Soares M.B., Bonaldo M.F., Casavant T.L., Scheetz T.E.,
RA Brownstein M.J., Usdin T.B., Toshiyuki S., Carninci P., Prange C.,
RA Raha S.S., Loquellano N.A., Peters G.J., Abramson R.D., Mullahy S.J.,
RA Bosak S.A., McEwan P.J., McKernan K.J., Malek J.A., Gunaratne P.H.,
RA Richards S., Worley K.C., Hale S., Garcia A.M., Gay L.J., Hulyk S.W.,
RA Villalon D.K., Muzny D.M., Sodergren E.J., Lu X., Gibbs R.A.,
RA Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., Sanchez A.,
RA Whiting M., Madan A., Young A.C., Shevchenko Y., Bouffard G.G.,
RA Blakesley R.W., Touchman J.W., Green E.D., Dickson M.C.,
RA Rodriguez A.C., Grimwood J., Schmutz J., Myers R.M.,
RA Butterfield Y.S.N., Krzywinski M.I., Skalska U., Smailus D.E.,
RA Schnerch A., Schein J.E., Jones S.J.M., Marra M.A.;
RT "Generation and initial analysis of more than 15,000 full-length human
RT and mouse cDNA sequences.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:16899-16903(2002).
RN [7]
RP GLYCOSYLATION.
RX MEDLINE=20266405; PubMed=10792058 [NCBI, ExPASy, EBI, Israel, Japan]; DOI=10.1073/pnas.100118597;
RA Meera P., Wallner M., Toro L.;
RT "A neuronal beta subunit (KCNMB4) makes the large conductance,
RT voltage- and Ca2+-activated K+ channel resistant to charybdotoxin and
RT iberiotoxin.";
RL Proc. Natl. Acad. Sci. U.S.A. 97:5562-5567(2000).
RN [8]
RP E2-BINDING.
RX PubMed=10489376 [NCBI, ExPASy, EBI, Israel, Japan]; DOI=10.1126/science.285.5435.1929;
RA Valverde M.A., Rojas P., Amigo J., Cosmelli D., Orio P.,
RA Bahamonde M.I., Mann G.E., Vergara C., Latorre R.;
RT "Acute activation of Maxi-K channels (hSlo) by estradiol binding to
RT the beta subunit.";
RL Science 285:1929-1931(1999).
RN [9]
RP REVIEW.
RX PubMed=12136044 [NCBI, ExPASy, EBI, Israel, Japan];
RA Orio P., Rojas P., Ferreira G., Latorre R.;
RT "New disguises for an old channel: MaxiK channel beta-subunits.";
RL News Physiol. Sci. 17:156-161(2002).
RN [10]
RP VARIANT LYS-64.
RX PubMed=15057310 [NCBI, ExPASy, EBI, Israel, Japan]; DOI=10.1172/JCI200420347;
RA Fernandez-Fernandez J.M., Tomas M., Vazquez E., Orio P., Latorre R.,
RA Senti M., Marrugat J., Valverde M.A.;
RT "Gain-of-function mutation in the KCNMB1 potassium channel subunit is
RT associated with low prevalence of diastolic hypertension.";
RL J. Clin. Invest. 113:1032-1039(2004).
CC -!- FUNCTION: Regulatory subunit of the calcium activated potassium
CC KCNMA1 (maxiK) channel. Modulates the calcium sensitivity and
CC gating kinetics of KCNMA1, thereby contributing to KCNMA1 channel
CC diversity. Increases the apparent Ca(2+)/voltage sensitivity of
CC the KCNMA1 channel. It also modifies KCNMA1 channel kinetics and
CC alters its pharmacological properties. It slows down the
CC activation and the deactivation kinetics of the channel. Acts as a
CC negative regulator of smooth muscle contraction by enhancing the
CC calcium sensitivity to KCNMA1. Its presence is also a requirement
CC for internal binding of the KCNMA1 channel opener
CC dehydrosoyasaponin I (DHS-1) triterpene glycoside and for external
CC binding of the agonist hormone 17-beta-estradiol (E2). Increases
CC the binding activity of charybdotoxin (CTX) toxin to KCNMA1
CC peptide blocker by increasing the CTX association rate and
CC decreasing the dissociation rate.
CC -!- SUBUNIT: Interacts with KCNMA1 tetramer. There are probably 4
CC molecules of KCMNB1 per KCNMA1 tetramer.
CC -!- SUBCELLULAR LOCATION: Integral membrane protein.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q16558-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q16558-2; Sequence=VSP_009822, VSP_009823;
CC Note=No experimental confirmation available;
CC -!- TISSUE SPECIFICITY: Abundantly expressed in smooth muscle. Low
CC levels of expression in most other tissues. Within the brain,
CC relatively high levels found in hippocampus and corpus callosum.
CC -!- PTM: N-glycosylated.
CC -!- POLYMORPHISM: Genetic variation in KCNMB1 can influence the
CC severity of diastolic hypertension.
CC -!- SIMILARITY: Belongs to the KCNMB family.
CC --------------------------------------------------------------------------
CC This Swiss-Prot entry is copyright. It is produced through a collaboration
CC between the Swiss Institute of Bioinformatics and the EMBL outstation -
CC the European Bioinformatics Institute. There are no restrictions on its
CC use as long as its content is in no way modified and this statement is not
CC removed.
CC --------------------------------------------------------------------------
DR EMBL; U25138; AAB02394.1; -; mRNA. [EMBL / GenBank / DDBJ] [CoDingSequence]
DR EMBL; U38907; AAA81327.1; -; mRNA. [EMBL / GenBank / DDBJ] [CoDingSequence]
DR EMBL; U42600; AAB16827.1; -; mRNA. [EMBL / GenBank / DDBJ] [CoDingSequence]
DR EMBL; U42601; AAB16825.1; -; Genomic_DNA. [EMBL / GenBank / DDBJ] [CoDingSequence]
DR EMBL; U42603; AAB16826.1; -; Genomic_DNA. [EMBL / GenBank / DDBJ] [CoDingSequence]
DR EMBL; U42602; AAB16826.1; JOINED; Genomic_DNA. [EMBL / GenBank / DDBJ] [CoDingSequence]
DR EMBL; U61536; AAB61396.1; -; mRNA. [EMBL / GenBank / DDBJ] [CoDingSequence]
DR EMBL; U61537; AAB61397.1; -; Genomic_DNA. [EMBL / GenBank / DDBJ] [CoDingSequence]
DR EMBL; AF026002; AAB88805.1; -; mRNA. [EMBL / GenBank / DDBJ] [CoDingSequence]
DR EMBL; AY044441; AAK95827.1; -; mRNA. [EMBL / GenBank / DDBJ] [CoDingSequence]
DR EMBL; BC025707; AAH25707.1; -; mRNA. [EMBL / GenBank / DDBJ] [CoDingSequence]
DR PIR; S68842; S68842.
DR Ensembl; ENSG00000145936; Homo_sapiens
DR HGNC; HGNC:6285; KCNMB1.
DR CleanEx; HGNC:6285; KCNMB1.
DR MIM; 603951; -. [NCBI / EBI]
DR GeneCards; KCNMB1.
DR GeneLynx; KCNMB1.
DR GenAtlas; KCNMB1.
DR SOURCE; KCNMB1.
DR MIM; 608622; -. [NCBI / EBI]
DR GO; GO:0015269; F:calcium-activated potassium channel activity; TAS.
DR GO; GO:0015459; F:potassium channel regulator activity; TAS.
DR GO; GO:0006813; P:potassium ion transport; TAS.
DR GO; GO:0007268; P:synaptic transmission; TAS.
DR InterPro; IPR003930; BK_channel_beta.
DR InterPro; Graphical view of domain structure.
DR PANTHER; PTHR10258; BK_channel_beta; 1.
DR Pfam; PF03185; CaKB; 1.
DR Pfam; Graphical view of domain structure.
DR PRINTS; PR01450; BKCHANNELB.
DR CMR; Q16558.
DR ProDom [Domain structure / List of seq. sharing at least 1 domain]
DR HOVERGEN [Family / Alignment / Tree]
DR BLOCKS; Q16558.
DR ProtoNet; Q16558.
DR ProtoMap; Q16558.
DR PRESAGE; Q16558.
DR DIP; Q16558.
DR ModBase; Q16558.
DR SWISS-2DPAGE; GET REGION ON 2D PAGE.
KW Alternative splicing; Glycoprotein; Ion transport; Ionic channel;
KW Polymorphism; Transmembrane; Transport.
FT INIT_MET 0 0 By similarity.
FT TOPO_DOM 1 17 Cytoplasmic (Potential).
FT TRANSMEM 18 38 1 (Potential).
FT TOPO_DOM 39 156 Extracellular (Potential).
FT TRANSMEM 157 177 2 (Potential).
FT TOPO_DOM 178 190 Cytoplasmic (Potential).
FT CARBOHYD 79 79 N-linked (GlcNAc...) (Potential).
FT CARBOHYD 141 141 N-linked (GlcNAc...) (Potential).
FT VARSPLIC 102 129 CSYIPGSVDNYQTARADVEKVRAKFQEQ -> VLNWRDGDT
FT SLYPCQVCEPVPNCPCPRG (in isoform 2).
FT /FTId=VSP_009822.
FT VARSPLIC 130 190 Missing (in isoform 2).
FT /FTId=VSP_009823.
FT VARIANT 64 64 E -> K (has a protective effect against
FT diastolic hypertension; dbSNP:11739136).
FT /FTId=VAR_019325.
SQ SEQUENCE 190 AA; 21667 MW; 6DE23A70EF08A2B0 CRC64;
VKKLVMAQKR GETRALCLGV TMVVCAVITY YILVTTVLPL YQKSVWTQES KCHLIETNIR
DQEELEGKKV PQYPCLWVNV SAAGRWAVLY HTEDTRDQNQ QCSYIPGSVD NYQTARADVE
KVRAKFQEQQ VFYCFSAPRG NETSVLFQRL YGPQALLFSL FWPTFLLTGG LLIIAMVKSN
QYLSILAAQK
//