KChannelDB: Extraction of mutation data from the literature

2005, KChannelDB.

This data was extracted from Medline abstracts and full texts (when available) in an automated manner.

The table below describes the selected point mutation and provides links to other documents. The sentence(s) where the point mutation K185E was found are listed after the table.

The mutated residues are also indicated in the family sequence alignments and hyperlinked to the corresponding mutation pages.

Point mutation K185E in IRK11_MOUSE

Point mutation:	K185E
Domain:	C-term
General numbering (KChannelDB):	-
Protein:	IRK11_MOUSE (Kcnj1)	Swiss-Prot Cross-reference table Family page
Other point mutations / same protein / same position	Point mutations at position 185 in IRK11_MOUSE
Other point mutations / same protein	List of mutations in IRK11_MOUSE
Family alignments	Inward rectifiers (Kir) Potassium channels 2 TMs
Other point mutations / same position	Position 179 in Inward rectifiers (Kir) family Position 179 in Potassium channels 2 TMs family
Reference:	Interaction of stilbene disulphonates with cloned K(ATP) channels. Proks P, Jones P, Ashcroft FM Br J Pharmacol 2001 Mar;132(5):973-82.	Medline
Other point mutations / same article	List
Text source	HTML full text
Validation status	Not yet checked

Relevant sentences:

K185E

To confirm that the site at which ATP protects against DIDS inhibition is the same as that at which ATP mediates channel inhibition , we examined a mutant form of Kir6.2 (Kir6.2{Delta}C-K185E) that shows reduced ATP sensitivity (Reimann et al. , 1999(image) ) and decreased ATP binding (Tanabe et al. , 1999(image) )
In contrast to the wild-type channel , ATP did not protect against DIDS block of Kir6.2{Delta}C-K185E currents (Figure 2C )
The time constant of DIDS block was significantly (P<0.001) slowed: from 4.4±1.5 s (n = 6) for the wild-type Kir6.2{Delta}C to 18.3±3.2 s (n = 5) for the Kir6.2{Delta}C-K185E mutant
This may explain why some mutations slowed the time-course of channel inhibition (e.g. mutation K185E , Figure 2C )

F.Horn (kchanneldbcmbi.ru.nl), 17-Aug-2005