KChannelDB: Extraction of mutation data from the literature

2005, KChannelDB.

This data was extracted from Medline abstracts and full texts (when available) in an automated manner.

The table below describes the selected point mutation and provides links to other documents. The sentence(s) where the point mutation I229L was found are listed after the table.

The mutated residues are also indicated in the family sequence alignments and hyperlinked to the corresponding mutation pages.

Point mutation I229L in IRK5_RAT

Point mutation:	I229L
Domain:	C-term
General numbering (KChannelDB):	-
Protein:	IRK5_RAT (Kcnj5,Girk)	Swiss-Prot Cross-reference table Family page
Other point mutations / same protein	List of mutations in IRK5_RAT
Family alignments	Inward rectifiers (Kir) Potassium channels 2 TMs
Other point mutations / same position	Position 204 in Inward rectifiers (Kir) family Position 204 in Potassium channels 2 TMs family
Reference:	Molecular basis for the inhibition of G protein-coupled inward rectifier K(+) channels by protein kinase C. Mao J, Wang X, Chen F, Wang R, Rojas A, Shi Y, Piao H, Jiang C Proc Natl Acad Sci U S A 2004 Jan 27;101(4):1087-92. Epub 2004 Jan 19.	Medline
Other point mutations / same article	List
Text source	HTML full text
Validation status	Not yet checked

Relevant sentences:

I229L

To express detectable GIRK4*S227A and GIRK4*S321A currents , another mutation of I229L , which has been previously used to rescue nonfunctional GIRK mutants by enhancing the channel-PIP2 interaction (14 , 42 ) , was introduced into these two mutants
The GIRK4*I229L / S321A showing larger basal currents was still inhibited by PMA to the same degree as the GIRK4*I229L (59.9 ± 3.7% , n = 10 for GIRK4*I229L ; and 59.6 ± 3.9% , n = 9 for GIRK4*I229L-S321A) , suggesting that Ser-321 in GIRK4 is not a PKC phosphorylation site
There was no evident expression of inward rectifying currents by injection of the GIRK4*I229L / S227A mutant , either by coinjections of GIRK4*S227A with the cDNAs encoding G beta gamma
We also examined the SP sensitivity of the GIRK4*I229L expressed with the NK1R
Because PMA inhibits the GIRK4*I229L [a mutant defective in PIP2 sensitivity (42 )] and because PMA at µM concentrations decreases PIP2 (55 ) , PIP2 depletion may have rather small contribution to the GIRK channel inhibition by nM PMA
Also , SP inhibits the GIRK4*I229L mutant

F.Horn (kchanneldbcmbi.ru.nl), 17-Aug-2005