KChannelDB: Extraction of mutation data from the literature

2005, KChannelDB.

This data was extracted from Medline abstracts and full texts (when available) in an automated manner.

The table below describes the selected point mutation and provides links to other documents. The sentence(s) where the point mutation R171D was found are listed after the table.

The mutated residues are also indicated in the family sequence alignments and hyperlinked to the corresponding mutation pages.

Point mutation R171D in KAT1_ARATH

Point mutation:	R171D
Domain:	TRANSMEM IV
General numbering (KChannelDB):	450
Protein:	KAT1_ARATH (KAT1,At5g46240,MPL12.)	Swiss-Prot Cross-reference table Family page
Other point mutations / same protein / same position	Point mutations at position 171 in KAT1_ARATH
Other point mutations / same protein	List of mutations in KAT1_ARATH
Family alignments	AKT-like Inward rectifiers 6TMs Plant potassium channels
Other point mutations / same position	Position 173 in AKT-like Inward rectifiers 6TMs family Position 173 in Plant potassium channels family
Reference:	Molecular dissection of the contribution of negatively and positively charged residues in S2, S3, and S4 to the final membrane topology of the voltage sensor in the K+ channel, KAT1. Sato Y, Sakaguchi M, Goshima S, Nakamura T, Uozumi N J Biol Chem 2003 Apr 11;278(15):13227-34. Epub 2003 Jan 29.	Medline
Other point mutations / same article	List
Text source	HTML full text
Validation status	Not yet checked

Relevant sentences:

R171D

The charge reversal mutation , R171D , in S4 rescued the D105R mutation and prevented S4 release into the ER lumen
D , membrane topology of double mutants containing D105R plus R165D , R171D , R174D , R176D , or R177D
E , membrane topology of the R171D mutant
As shown in Fig. 4 D , the four D105R mutants containing R165D , R174D , R176D , or R177D gave a mixture of mono- and di-glycosylated bands , whereas that containing R171D gave only the mono-glycosylated band
Moreover , we confirmed that the R171D mutation alone (without D105R) led to some translocation of S4 into the ER lumen (Fig. 4 E)

F.Horn (kchanneldbcmbi.ru.nl), 17-Aug-2005