KChannelDB: Extraction of mutation data from the literature

2005, KChannelDB.

This data was extracted from Medline abstracts and full texts (when available) in an automated manner.

The table below describes the selected point mutation and provides links to other documents. The sentence(s) where the point mutation H116Q was found are listed after the table.

The mutated residues are also indicated in the family sequence alignments and hyperlinked to the corresponding mutation pages.

Point mutation H116Q in IRK3_RAT

Point mutation:	H116Q
Domain:	Loop 1-2
General numbering (KChannelDB):	-
Protein:	IRK3_RAT (Kcnj3,Girk1, Kg)	Swiss-Prot Cross-reference table Family page
Other point mutations / same protein	List of mutations in IRK3_RAT
Family alignments	Inward rectifiers (Kir) Potassium channels 2 TMs
Other point mutations / same position	Position 97 in Inward rectifiers (Kir) family Position 96 in Potassium channels 2 TMs family
Reference:	Molecular determinants for activation of G-protein-coupled inward rectifier K+ (GIRK) channels by extracellular acidosis. Mao J, Li L, McManus M, Wu J, Cui N, Jiang C J Biol Chem 2002 Nov 29;277(48):46166-71.	Medline
Other point mutations / same article	List
Text source	HTML full text
Validation status	Not yet checked

Relevant sentences:

H116Q

Such activation was eliminated when a histidine residue in the M1-H5 linker was mutated to a non-titratable glutamine , i.e. H116Q in GIRK1 and H120Q in GIRK4
We found that heteromeric channels carrying any one of the histidines (GIRK1-H116Q / GIRK4 , GIRK1 / GIRK4-H120Q) remained pH o-sensitive , although their pH o sensitivity was significantly lower than the wild-type channels
Simultaneous mutations of the histidine in both GIRK1 and GIRK4 (GIRK1H116Q-GIRK4H120Q) completely eliminated the pH o sensitivity of the heteromeric channels (Figs
A , there was no change in the current amplitude in the GIRK1-H116Q / GIRK4-H120Q when pH o was reduced from 7.4 to 6.2
B , the GIRK1-H116Q / GIRK4-H120Q mutant channel did not respond to a range of pH o changes from 8.4 to 6.2

F.Horn (kchanneldbcmbi.ru.nl), 17-Aug-2005