KChannelDB: Extraction of mutation data from the literature

2005, KChannelDB.

This data was extracted from Medline abstracts and full texts (when available) in an automated manner.

The table below describes the selected point mutation and provides links to other documents. The sentence(s) where the point mutation G628C was found are listed after the table.

The mutated residues are also indicated in the family sequence alignments and hyperlinked to the corresponding mutation pages.

Point mutation G628C in KCNH2_HUMAN

Point mutation:	G628C
Domain:	Loop 5-6
General numbering (KChannelDB):	-
Protein:	KCNH2_HUMAN (KCNH2,ERG, ERG1, HERG, HERG)	Swiss-Prot Cross-reference table Family page
Protein isoforms	4
Other point mutations / same protein / same position	Point mutations at position 628 in KCNH2_HUMAN
Other point mutations / same protein	List of mutations in KCNH2_HUMAN
Family alignments	eag related KCNH (Kv10-12) K+ voltage-gated channels (Kv1-12,Kca2-5) Potassium channels 6 TMs
Other point mutations / same position	Position 628 in eag related KCNH (Kv10-12) family Position 616 in K+ voltage-gated channels (Kv1-12,Kca2-5) family Position 376 in Potassium channels 6 TMs family
Reference:	Open channel block of HERG K(+) channels by vesnarinone. Kamiya K, Mitcheson JS, Yasui K, Kodama I, Sanguinetti MC Mol Pharmacol 2001 Aug;60(2):244-53.	Medline
Other point mutations / same article	List
Text source	HTML full text
Validation status	Not yet checked

Relevant sentences:

G628C

Vesnarinone produced similar effects on inactivation-removed mutant (G628C / S631C) HERG channels
C-type inactivation of HERG channels can be removed through the simultaneous introduction of two point mutations (G628C , S631C) (Smith et al. , 1996(image) )
Stage V and VI oocytes were injected with 10 ng of cRNA encoding wild-type (WT) HERG or G628C:S631C HERG
Therefore , we determined the concentration-dependence for block of G628C / S631C HERG channel current by vesnarinone
The outward G628C / S631C HERG channel current was elicited by the same protocol used to evaluate WT currents (Fig. 1 )
The inhibition of G628C / S631C HERG channel current was not voltage-dependent (Fig. 6 C and D)
Block of inactivation-removed (G628C / S631C) HERG channel current by vesnarinone
Because tail currents were too small to analyze , time-dependent changes of G628C / S631C HERG channel block by vesnarinone were evaluated during a 2-s depolarizing pulse (Fig. 7 A)
Recovery from block of G628C / S631C HERG current was evaluated at different hyperpolarizing levels using a paired-pulse protocol (Fig. 7 D)
Thus , similar to WT HERG , the recovery of G628C / S631C HERG current block by vesnarinone was only weakly voltage-dependent
Onset and recovery of G628C / S631C HERG channel current block by vesnarinone assessed during and after depolarizing voltage steps
A , representative recordings of G628C / S631C HERG currents elicited by 2-s pulses to +50 mV before and after 100 然 vesnarinone
Therefore , relative step currents were estimated by the ratio of G628C / S631C HERG currents before and after application of vesnarinone
B , time constants for the onset of G628C / S631C HERG current block at different voltages for 30 然 (triangle ) and 100 然 (black-triangle) vesnarinone (n = 5)
D , recovery from block of G628C / S631C HERG channel current by vesnarinone at hyperpolarized holding potentials
E , time constants for recovery from block of G628C / S631C HERG current by 100 然 vesnarinone (n = 4)
Moreover , we found that the drug was equally effective in blocking WT and inactivation-removed (G628C / S631C) HERG channels

F.Horn (kchanneldbcmbi.ru.nl), 17-Aug-2005