KChannelDB: Extraction of mutation data from the literature 2005, KChannelDB.
This data was extracted from Medline abstracts and full texts (when available) in an automated manner.
The table below describes the selected point mutation and provides links to other documents. The sentence(s) where the point mutation G628C was found are listed after the table.
The mutated residues are also indicated in the family sequence alignments and hyperlinked to the corresponding mutation pages.
Point mutation G628C in KCNH2_HUMAN
|General numbering (KChannelDB):|| -|
|Protein:||KCNH2_HUMAN (KCNH2,ERG, ERG1, HERG, HERG)||Swiss-Prot|
|Other point mutations / same protein / same position||Point mutations at position 628 in KCNH2_HUMAN|
|Other point mutations / same protein||List of mutations in KCNH2_HUMAN|
eag related KCNH (Kv10-12)|
K+ voltage-gated channels (Kv1-12,Kca2-5)
Potassium channels 6 TMs
|Other point mutations / same position||
Position 628 in eag related KCNH (Kv10-12) family |
Position 616 in K+ voltage-gated channels (Kv1-12,Kca2-5) family
Position 376 in Potassium channels 6 TMs family
|Reference:||Open channel block of HERG K(+) channels by vesnarinone.|
Kamiya K, Mitcheson JS, Yasui K, Kodama I, Sanguinetti MC
Mol Pharmacol 2001 Aug;60(2):244-53.
|Other point mutations / same article||List|
|Text source||HTML full text|
|Validation status||Not yet checked|
- Vesnarinone produced similar effects on inactivation-removed mutant (G628C / S631C) HERG channels
- C-type inactivation of HERG channels can be removed through the simultaneous introduction of two point mutations (G628C , S631C) (Smith et al. , 1996(image) )
- Stage V and VI oocytes were injected with 10 ng of cRNA encoding wild-type (WT) HERG or G628C:S631C HERG
- Therefore , we determined the concentration-dependence for block of G628C / S631C HERG channel current by vesnarinone
- The outward G628C / S631C HERG channel current was elicited by the same protocol used to evaluate WT currents (Fig. 1 )
- The inhibition of G628C / S631C HERG channel current was not voltage-dependent (Fig. 6 C and D)
- Block of inactivation-removed (G628C / S631C) HERG channel current by vesnarinone
- Because tail currents were too small to analyze , time-dependent changes of G628C / S631C HERG channel block by vesnarinone were evaluated during a 2-s depolarizing pulse (Fig. 7 A)
- Recovery from block of G628C / S631C HERG current was evaluated at different hyperpolarizing levels using a paired-pulse protocol (Fig. 7 D)
- Thus , similar to WT HERG , the recovery of G628C / S631C HERG current block by vesnarinone was only weakly voltage-dependent
- Onset and recovery of G628C / S631C HERG channel current block by vesnarinone assessed during and after depolarizing voltage steps
- A , representative recordings of G628C / S631C HERG currents elicited by 2-s pulses to +50 mV before and after 100 ÁM vesnarinone
- Therefore , relative step currents were estimated by the ratio of G628C / S631C HERG currents before and after application of vesnarinone
- B , time constants for the onset of G628C / S631C HERG current block at different voltages for 30 ÁM (triangle ) and 100 ÁM (black-triangle) vesnarinone (n = 5)
- D , recovery from block of G628C / S631C HERG channel current by vesnarinone at hyperpolarized holding potentials
- E , time constants for recovery from block of G628C / S631C HERG current by 100 ÁM vesnarinone (n = 4)
- Moreover , we found that the drug was equally effective in blocking WT and inactivation-removed (G628C / S631C) HERG channels
F.Horn (kchanneldbcmbi.ru.nl), 17-Aug-2005